Temperature-dependent growth rates and gene expression patterns of various medaka Oryzias latipes cell lines derived from different populations
Temperature-dependent growth rates and gene expression patterns of various medaka Oryzias latipes cell lines derived from different populations
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DOI：
10.1007/s00360-005-0053-8
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发表时间：
2006-05-01
影响因子：
2
通讯作者：
Watabe, S
中科院分区：
文献类型：
--
作者：
Hirayama, M;Mitani, H;Watabe, S
青鳉Oryzias latipes有几个地理和遗传上不同的种群。我们研究了来自不同种群的不同青鳉细胞系的温度适应反应。在不同温度下的细胞生长的测量表明，15摄氏度是允许生长的温度在所有细胞系从北方日本和韩国东部的人口，但不是在那些从日本南部人口和青鳉相关的物种Oryzias celebensis，居住在热带地区。RT-PCR的102个温度响应基因，以前在其他物种中报道，揭示了编码HSP 47的基因的积累的mRNA水平在25摄氏度低于在33摄氏度，反之亦然，包括I κ B α和Ra B-1c，在OLHNI-1细胞系从北方日本人口。通过实时PCR的进一步分析表明，当培养温度从33 ℃转变为15 ℃时，来自韩国东部人群的OLHNI-1和OLSOK-e7细胞系中I κ B α和Ra B-1c的累积mRNA水平增加，但在来自日本南部人群的OLHdrR-e3细胞系中则没有。由于I kappa B α和Ra B-1c分别与NF kappa B级联和内质网-高尔基体转运相关，因此推断免疫应答和细胞内转运可能对青鳉的温度适应至关重要。
Medaka Oryzias latipes has several geographically and genetically distinct populations. We examined temperature acclimation response in various medaka cell lines derived from different populations. Measurement of cell growth at various temperatures suggested that 15 degrees C was the permissive growth temperature in all cell lines from the Northern Japanese and East Korean populations, but not in those from the Southern Japanese population and medaka-related species Oryzias celebensis, which inhabits a tropical zone. RT-PCR for 102 temperature-responsive genes, previously reported in other species, revealed that the accumulated mRNA level of a gene encoding HSP47 was lower at 25 degrees C than at 33 degrees C, and vice versa for 12 genes including I kappa B alpha and Rab-1c, in OLHNI-1 cell line from the Northern Japanese population. Further analysis by real-time PCR demonstrated that the accumulated mRNA levels of I kappa B alpha and Rab-1c in OLHNI-1 and OLSOK-e7 cell lines from the East Korean population were increased when the culture temperature was shifted from 33 to 15 degrees C, but not in OLHdrR-e3 cell line from the Southern Japanese population. Since I kappa B alpha and Rab-1c are related to the NF kappa B cascade and endoplasmic reticulum-to-Golgi transport, respectively, it is inferred that immune responses and intracellular transport are possibly critical to temperature adaptation for medaka.