Postnatal reorganization of primary afferent terminal fields in the rat gustatory brainstem is determined by prenatal dietary history
Postnatal reorganization of primary afferent terminal fields in the rat gustatory brainstem is determined by prenatal dietary history
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DOI：
10.1002/cne.21760
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发表时间：
2008-08-20
影响因子：
2.5
通讯作者：
Hill, David L.
中科院分区：
文献类型：
--
作者：
Mangold, Jamie E.;Hill, David L.
饮食操纵已被用作一种实验策略，以了解味觉系统的正常发展。从胚胎第3天（E3）到E12（E3-E12钠限制大鼠）的低氯化钠饮食制度在成年期产生了鼓索（CT）、岩浅大神经（GSP）和孤束核（NTS）吻极中舌咽神经（IX）的显著扩大的终末野。为了研究这种早期的、有限的饮食操作如何影响出生后终末野的发育，我们在出生后第15天（P15）、P25、P35和>= P40（成年人）对两组大鼠进行了三重顺行神经标记程序：喂饲含钠商业饮食的大鼠（对照组）和E3-E12钠限制的大鼠。结果显示，在正常发育过程中，所有三种神经的终末野体积均随年龄增长而减少。相反，E3-E12钠限制大鼠显示CT和IX终末场的年龄相关性增加，GSP终末场体积在整个发育过程中保持不变。NTS体积没有增长后P15，因此，终端领域的体积的变化是不是由于平行的NTS的大小的变化。我们的数据表明，在控制中观察到的终端领域的年龄相关的减少可能反映了活动依赖性修剪的传入终端，而终端领域的E3-E12钠限制大鼠中看到的增加可能反映主要由活动独立的机制诱导的NTS的细胞/分子差异。这些发现预示着E3-E12钠限制大鼠和对照组之间的神经编码和感觉引导行为的发展存在显着差异。
Dietary manipulation has been used as an experimental strategy to gain insight into the normal development of the gustatory system. Institution of a diet low in sodium chloride (NaCl) from embryonic day 3 (E3) to E 12 (E3-E12 sodium-restricted rats) yields dramatically enlarged terminal fields of the chorda tympani (CT), greater superficial petrosal (GSP), and glossopharyngeal (IX) nerves in the rostral pole of the nucleus of the solitary tract (NTS) at adulthood. To examine how this early, limited dietary manipulation affects postnatal terminal field development, we used a triple anterograde nerve label procedure at postnatal day 15 (P15), P25, P35, and >= P40 (adults) in two groups: rats fed a commercial diet replete in sodium (controls) and E3-E12 sodium-restricted rats. Results showed an age-related decrease in terminal field volumes of all three nerves during normal development. In contrast, E3-E12 sodium-restricted rats displayed age-related increases of the CT and IX terminal fields, with the terminal field volume of the GSP remaining unchanged throughout development. NTS volume did not grow after P15; therefore, alterations in terminal field volumes are not due to parallel alterations in the size of the NTS. Our data suggest that the age-related decrease in terminal fields observed in controls may reflect activity-dependent pruning of afferent terminals, whereas terminal field increases seen in E3-E12 sodium-restricted rats may reflect cellular/molecular differences in the NTS induced predominantly by activity-independent mechanisms. These findings predict a significant difference in the development of neural coding and sensory-guided behaviors between E3-E12 sodium-restricted rats and controls.