GENETIC-ANALYSIS OF THE MLS SYSTEM - FORMAL MLS TYPING OF THE COMMONLY USED INBRED STRAINS
GENETIC-ANALYSIS OF THE MLS SYSTEM - FORMAL MLS TYPING OF THE COMMONLY USED INBRED STRAINS
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DOI：
10.1007/bf00211697
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发表时间：
1991-01-01
期刊：
影响因子：
3.2
通讯作者：
HODES, RJ
中科院分区：
文献类型：
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作者：
ABE, R;FOOPHILLIPS, M;HODES, RJ
为了阐明次要淋巴细胞刺激（Mls）基因产物的生物学作用，我们一直在研究Mls系统的基本免疫遗传学特征。 在本报告中，我们描述了刺激性Mls产物Mls（a）和Mls（c）在一组实验室近交系中的分布，这些近交系基于H-2相容性初始T细胞群体以及单特异性Mls（a）或Mls（c）反应性T细胞克隆的反应模式。 此外，检测了四种不同T细胞受体（Tcr）b-V片段Tcrb-V3、-V6、-V8.1和-V9的表达，这些片段最近被报道与这些菌株中Mls基因产物的T细胞识别相关。 结果表明，大多数常用的实验室菌株，包括最初分型为Mls（a）的菌株，也表达Mls（c）决定簇，极少数独立的近交系菌株是非Mls（c）。 此外，脾脏和胸腺中Tcrb-V的表达模式表明，Mls表达与自身Mls反应性T细胞的克隆缺失之间的关联似乎是近交系中的一般规则。 基于这些结果，不可检测的MLS样基因产物在其他物种除了小鼠的影响进行了讨论。
In order to elucidate the biological role of minor lymphocyte stimulating (Mls) gene products, we have been investigating the fundamental immunogenetic characteristics of the Mls system. In this report, we describe the distribution of stimulatory Mls products, Mls(a) and Mls(c), in a panel of laboratory inbred strains based on the response pattern of H-2-compatible naive T-cell populations as well as monospecific Mls(a)- or Mls(c)-reactive T-cell clones. In addition, the expression of four different T-cell receptor (Tcr) b-V segment Tcrb-V3, -V6, -V8.1, and -V9, which were recently reported to be associated with T-cell recognition of Mls gene products in these strains, was examined. The results indicate that the majority of commonly used laboratory strains including those originally typed as Mls(a) are also expressing Mls(c) determinants and that very few independent inbred strains are non-Mls(c). Moreover, the pattern of Tcrb-V expression in spleen as well as in thymus suggests that the association between Mls expression and clonal deletion of self Mls-reactive T cells appears to be the general rule in inbred strains. Based on these results, implications for the nondetectable Mls-like gene products in other species besides the mouse are discussed.