Systemic Antibody Responses to the Immunodominant p23 Antigen and p23 Polymorphisms in Children with Cryptosporidiosis in Bangladesh
Systemic Antibody Responses to the Immunodominant p23 Antigen and p23 Polymorphisms in Children with Cryptosporidiosis in Bangladesh
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DOI：
10.4269/ajtmh.2012.11-0273
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发表时间：
2012-02-01
影响因子：
3.3
通讯作者：
Ward, Honorine D.
中科院分区：
文献类型：
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作者：
Borad, Anoli J.;Allison, Geneve M.;Ward, Honorine D.
隐孢子虫是发展中国家儿童腹泻的主要原因。然而，目前还没有疫苗可用，对保护性抗原的免疫反应知之甚少。我们调查了孟加拉国患有隐孢子虫病和腹泻的儿童(病例)和未感染腹泻的儿童(对照)对p23的抗体反应，以及感染物种中p23基因的多态性。经过三周的随访，病例组的血清对p23的免疫球蛋白M、免疫球蛋白和免疫球蛋白A的反应显著高于对照组。急性腹泻者的血清IgA和IgM反应明显高于持续性腹泻者，这表明这与预防长期疾病有关。P23基因序列在感染种和亚型家系中相对保守。虽然大多数儿童感染了人隐孢子虫，但对微小隐孢子虫抗原有交叉反应的抗体反应。这些结果支持p23作为候选疫苗的进一步发展。
Cryptosporidium is a major cause of diarrhea in children in developing countries. However, there is no vaccine available and little is known about immune responses to protective antigens. We investigated antibody responses to p23, a putative vaccine candidate, in children in Bangladesh with cryptosporidiosis and diarrhea (cases) and uninfected children with diarrhea (controls), and p23 gene polymorphisms in infecting species. Serum IgM, IgG, and IgA responses to p23 were significantly greater in cases than controls after three weeks of follow-up. Cases with acute diarrhea had significantly greater serum IgA and IgM responses than those with persistent diarrhea, which suggested an association with protection from prolonged disease. The p23 sequences were relatively conserved among infecting species and subtype families. Although most children were infected with Cryptosporidium hominis, there was a cross-reactive antibody response to C. parvum antigen. These results support further development of p23 as a vaccine candidate.