Transcriptome profiling confirmed correlations between symptoms and transcriptional changes in RDV infected rice and revealed nucleolus as a possible target of RDV manipulation.
Transcriptome profiling confirmed correlations between symptoms and transcriptional changes in RDV infected rice and revealed nucleolus as a possible target of RDV manipulation.
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转录组分析证实了 RDV 感染水稻的症状与转录变化之间的相关性，并揭示了核仁是 RDV 操纵的可能目标
DOI：
10.1186/1743-422x-11-81
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发表时间：
2014-05-06
期刊：
影响因子：
4.8
通讯作者：
Wu J
中科院分区：
文献类型：
--
作者：
Yang L;Du Z;Gao F;Wu K;Xie L;Li Y;Wu Z;Wu J
水稻矮缩病毒（Rice dwarf virus，RDV）是引起水稻矮缩病的病原，严重限制了东南亚许多地区的水稻生产。Shimizu et al.和Satoh et al.已经表征了水稻响应RDV感染的转录变化。这两项研究都发现了防御相关基因的诱导以及转录变化与RDV感染水稻症状发展之间的相关性。然而，在两项研究中使用了相同的水稻品种，即属于水稻的日本亚种的日本晴。水稻籼亚种的基因表达变化。SSP.利用亲和水稻基因组阵列对中等抗RDV的籼稻品种宜香2292进行了鉴定。差异表达基因（DEG）根据其基因本体（GO）注释进行分类。利用实时荧光定量PCR（RT-PCR）技术研究了Pns 11基因在本氏烟草（Nicotiana benthaminana）中瞬时表达对核仁基因表达的影响。856个参与防御或其他生理过程的基因被鉴定为DEG，其中大部分表达上调。核糖体和核仁相关基因在DEG中显著富集。与核仁功能相关的代表性基因在N.瞬时表达RDV的Pns 11的benthaminana植物。水稻感染RDV后，防御相关基因的诱导是常见的。RDV感染水稻后，症状严重程度与转录水平的变化存在相关性。除了核糖体外，RDV还可能以核仁为靶点来操纵水稻的翻译机制。鉴于核仁和核糖体之间的紧密联系，推测RDV可能通过Pns 11靶向核仁来增强核糖体基因的表达是有趣的。
Rice dwarf virus (RDV) is the causal agent of rice dwarf disease, which limits rice production in many areas of south East Asia. Transcriptional changes of rice in response to RDV infection have been characterized by Shimizu et al. and Satoh et al.. Both studies found induction of defense related genes and correlations between transcriptional changes and symptom development in RDV-infected rice. However, the same rice cultivar, namely Nipponbare belonging to the Japonic subspecies of rice was used in both studies. Gene expression changes of the indica subspecies of rice, namely Oryza sativa L. ssp. indica cv Yixiang2292 that show moderate resistance to RDV, in response to RDV infection were characterized using an Affymetrix Rice Genome Array. Differentially expressed genes (DEGs) were classified according to their Gene Ontology (GO) annotation. The effects of transient expression of Pns11 in Nicotiana benthaminana on the expression of nucleolar genes were studied using real-time PCR (RT-PCR). 856 genes involved in defense or other physiological processes were identified to be DEGs, most of which showed up-regulation. Ribosome- and nucleolus related genes were significantly enriched in the DEGs. Representative genes related to nucleolar function exhibited altered expression in N. benthaminana plants transiently expressing Pns11 of RDV. Induction of defense related genes is common for rice infected with RDV. There is a co-relation between symptom severity and transcriptional alteration in RDV infected rice. Besides ribosome, RDV may also target nucleolus to manipulate the translation machinery of rice. Given the tight links between nucleolus and ribosome, it is intriguing to speculate that RDV may enhance expression of ribosomal genes by targeting nucleolus through Pns11.