Growth Associated Protein-43 mRNA Expression in Nucleus Ambiguus Motoneurons after Recurrent Laryngeal Nerve Injury in the Rat
Growth Associated Protein-43 mRNA Expression in Nucleus Ambiguus Motoneurons after Recurrent Laryngeal Nerve Injury in the Rat
复制标题
大鼠喉返神经损伤后疑核运动神经元生长相关蛋白 43 mRNA 表达
作者：
T. Uno;K. Shogaki;H. Bamba;S. Koike;Y. Naruse;N. Ijima;Masaki Tanaka;Y. Hisa
目的：探讨喉返神经（RLN）横断后大鼠歧义核（NA）运动神经元生长相关蛋白43 (growth associated protein-43, GAP-43) mRNA表达的时间依赖性差异。材料与方法：动物于开腹后第1、7、14、21天处死。然后对这些切片进行GAP-43 mRNA的原位杂交处理。结果：gap -43 mRNA在开刀后短暂升高，在开刀后第7天达到峰值，在开刀后第14、21天逐渐下降。腋切开术后NA区GAP-43的表达高峰期与面核和舌下核的表达高峰期不同。结论：我们已经报道过，神经一氧化氮合酶（nNOS）在腋切开术后的NA运动神经元中被诱导，并在术后第14天达到峰值。本研究表明，GAP-43和nNOS的表达在时间上存在差异。
Objective—To investigate the time-dependent differences in growth associated protein-43 (GAP-43) mRNA expression in the nucleus ambiguus (NA) motoneurons after recurrent laryngeal nerve (RLN) transection in the rat. Material and Methods—Animals were sacrificed on Days 1, 7, 14 and 21 after axotomy. The sections were then processed for in situ hybridization of GAP-43 mRNA. Results—GAP-43 mRNA transiently increased after axotomy, reaching a peak on postoperative Day 7, and then decreased gradually at 14 and 21 days after axotomy. The peak period of GAP-43 expression in the NA is different from those in the facial and hypoglossal nuclei after axotomy. Conclusion—We have already reported that neuronal nitric oxide synthase (nNOS) is induced in the NA motoneurons after axotomy and reaches a peak on postoperative Day 14. This study shows that the expressions of GAP-43 and nNOS are chronologically different.