Pathogen‐driven coevolution across the CBP60 plant immune regulator subfamilies confers resilience on the regulator module
Pathogen‐driven coevolution across the CBP60 plant immune regulator subfamilies confers resilience on the regulator module
复制标题
CBP60 植物免疫调节子家族中病原体驱动的协同进化赋予调节器模块弹性
DOI：
10.1111/nph.17769
复制
发表时间：
2021
期刊：
影响因子：
9.4
通讯作者：
Katagiri, Fumiaki
中科院分区：
文献类型：
--
作者：
Zheng, Qi;Majsec, Kristina;Katagiri, Fumiaki
植物免疫信号网络的组成部分需要一种机制，赋予其抵御快速进化的病原体效应物的能力。在拟南芥CaM - Binding Protein (CBP) 60家族的8个成员中，AtCBP60g和AtSARD1是部分功能冗余的主要阳性免疫调节因子，而AtCBP60a是一个负性免疫调节因子。我们研究了CBP60a、CBP60g和SARD1免疫调节亚家族之间可能的弹性进化机制。系统发育分析研究了CBP60亚家族新功能化的次数。然后，利用基于新开发的欧几里得空间蛋白质进化分析（PEAES）分析平台的两两距离排序，对可能赋予调节模块弹性的特定协同进化机制的假设进行了检验。免疫调节亚家族在被子植物分化时期开始分化，并迅速进化。我们在所有12个不同的核心仙人掌物种谱系中检测到免疫调节亚家族之间显著的共同进化相互作用。共同进化的相互作用与假设的共同进化机制一致。尽管CBP60免疫调节剂亚家族的成员进化异常快，但它们在多样化后很长一段时间内都影响着彼此的进化，这种方式可能赋予免疫调节剂模块抵御快速进化的病原体效应物的弹性。
Components of the plant immune signaling network need mechanisms that confer resilience against fast‐evolving pathogen effectors that target them. Among eight Arabidopsis CaM‐Binding Protein (CBP) 60 family members, AtCBP60g and AtSARD1 are partially functionally redundant, major positive immune regulators, and AtCBP60a is a negative immune regulator. We investigated possible resilience‐conferring evolutionary mechanisms among the CBP60a, CBP60g and SARD1 immune regulatory subfamilies.Phylogenetic analysis was used to investigate the times of CBP60 subfamily neofunctionalization. Then, using the pairwise distance rank based on the newly developed analytical platform Protein Evolution Analysis in a Euclidean Space (PEAES), hypotheses of specific coevolutionary mechanisms that could confer resilience on the regulator module were tested.The immune regulator subfamilies diversified around the time of angiosperm divergence and have been evolving very quickly. We detected significant coevolutionary interactions across the immune regulator subfamilies in all of 12 diverse core eudicot species lineages tested. The coevolutionary interactions were consistent with the hypothesized coevolution mechanisms.Despite their unusually fast evolution, members across the CBP60 immune regulator subfamilies have influenced the evolution of each other long after their diversification in a way that could confer resilience on the immune regulator module against fast‐evolving pathogen effectors.