Modulation of the immune response by probiotic strains in a mouse model of gluten sensitivity
Modulation of the immune response by probiotic strains in a mouse model of gluten sensitivity
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DOI：
10.1016/j.cyto.2009.08.003
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发表时间：
2009-12-01
期刊：
影响因子：
3.8
通讯作者：
Rossi, Mauro
中科院分区：
文献类型：
--
作者：
D'Arienzo, Rossana;Maurano, Francesco;Rossi, Mauro
益生菌菌株在调节肠道相关淋巴组织的活动中发挥重要作用。阐明益生菌驱动免疫调节的机制可能有助于它们在特定免疫介导疾病或预防方面的治疗应用。在这项研究中，我们探讨了不同乳杆菌和乳酸双歧杆菌对表达人DQ8异源二聚体（一种与乳糜泻（CD）相关的HLA分子）的转基因小鼠的影响。对未成熟骨髓源树突状细胞（iBMDCs）的体外分析显示，所有菌株均上调表面B7-2 (CD86)，表明DC成熟，但强度不同。没有菌株在iBMDCs中诱导显著水平的1110或IL-12，而tnf - α的表达主要由副干酪乳杆菌和发酵乳杆菌引起。有趣的是，这些菌株也被发现在体内增加抗原特异性tnf - α的分泌，在用麸质成分麦胶蛋白粘膜免疫的小鼠中共同给予益生菌。总之，这些发现强调了益生菌在食物抗原敏感性小鼠模型中对先天免疫和适应性免疫发挥菌株特异性诱导而不是抑制作用的能力。2009爱思唯尔有限公司版权所有。
Probiotic strains play an important role in modulating activities in the gut-associated lymphoid tissue. Elucidation of the mechanisms that mediate probiotic-driven immunomodulation may facilitate their therapeutic application for specific immune-mediated diseases or for prophylaxis. In this study, we explored the effect of different Lactobacillus spp. and Bifidobacterium lactis in transgenic mice expressing the human DQ8 heterodimer, a HLA molecule linked to Celiac Disease (CD). In vitro analysis on immature bone marrow-derived dendritic cells (iBMDCs) showed that all strains up-regulated surface B7-2 (CD86), indicative of DC maturation, however, with different intensity. No strain induced appreciable levels of 1110 or IL-12 in iBMDCs, whereas TNF-alpha expression was essentially elicited by Lactobacillus paracasei and Lactobacillus fermentum. Interestingly, these strains were found also to increase the antigen-specific TNF-alpha secretion in vivo, following co-administration of probiotic bacteria in mice mucosally immunized with the gluten component gliadin. Together these findings highlighted the ability of probiotics to exert strain-specific inductive rather than suppressive effects both on the innate and adaptive immunity in a mouse model of food antigen sensitivity. (C) 2009 Elsevier Ltd. All rights reserved.