Coordination of robust single cell rhythms in the Arabidopsis circadian clock via spatial waves of gene expression
Coordination of robust single cell rhythms in the Arabidopsis circadian clock via spatial waves of gene expression
复制标题
DOI：
10.1101/208900
复制
发表时间：
2017-10
期刊：
影响因子：
7.7
通讯作者：
P. Gould;M. Domijan;Mark Greenwood;Isao T. Tokuda;Hannah Rees;László Kozma-Bognár;Anthony J W Hall;James C. W. Locke
中科院分区：
文献类型：
--
作者：
P. Gould;M. Domijan;Mark Greenwood;Isao T. Tokuda;Hannah Rees;László Kozma-Bognár;Anthony J W Hall;James C. W. Locke
拟南芥生物钟协调基因调控的昼夜周期。虽然多反馈回路电路已被证明可以产生24小时节律，但目前仍不清楚单个细胞中的时钟有多强大，或者整个工厂的时钟时序如何协调。在这里，我们检查时钟活动在单细胞水平在拟南芥幼苗几天。我们的数据揭示了强大的单细胞振荡，虽然disschedised。特别是，我们观察到两个时钟活动波;一个向下，一个向上。我们还发现了细胞与细胞之间的时钟耦合的证据，特别是在根尖。一个简单的模型表明，细胞间的耦合和我们测量的细胞间的周期差可以产生观察到的波。我们的研究结果揭示了植物生物钟的空间结构，并表明，与集中的哺乳动物时钟，时钟有多个协调点在拟南芥。
The Arabidopsis circadian clock orchestrates gene regulation across the day/night cycle. Although a multiple feedback loop circuit has been shown to generate the 24h rhythm, it remains unclear how robust the clock is in individual cells, or how clock timing is coordinated across the plant. Here we examine clock activity at the single cell level across Arabidopsis seedlings over several days. Our data reveal robust single cell oscillations, albeit desynchronised. In particular, we observe two waves of clock activity; one going down, and one up the root. We also find evidence of cell-to-cell coupling of the clock, especially in the root tip. A simple model shows that cell-to-cell coupling and our measured period differences between cells can generate the observed waves. Our results reveal the spatial structure of the plant circadian clock and suggest that unlike the centralised mammalian clock, the clock has multiple points of coordination in Arabidopsis.