Physicochemically functionalized carbon nanohorns for multi-dimensional cancer elimination
Physicochemically functionalized carbon nanohorns for multi-dimensional cancer elimination
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DOI：
10.1016/j.carbon.2015.05.077
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发表时间：
2016-02-01
期刊：
影响因子：
10.9
通讯作者：
Miyako, Eijiro
中科院分区：
文献类型：
--
作者：
Chechetka, Svetlana A.;Zhang, Minfang;Miyako, Eijiro
我们已经开发了基于叶酸（FA）和聚乙烯亚胺官能化的碳纳米角缀合物的多组分纳米材料，所述碳纳米角缀合物封装磁铁矿纳米颗粒。使用各种实验装置，包括动态光散射，热重分析，傅里叶变换红外光谱法，和紫外-可见-近红外（UV-Vis-NIR）光谱法的纳米缀合物进行了表征。使用NIR激光器和射频感应加热系统来评估纳米缀合物的发热能力。与对照相比，含有纳米缀合物的水溶液在激光照射和/或射频诱导后表现出温度的显著增加。使用UV-Vis-NIR光谱对两种细胞系：人表皮癌（KB）和人胚胎（FHs 173 We）细胞的纳米缀合物的细胞间分布进行定量分析。FA修饰的纳米缀合物和KB细胞之间的相互作用通过KB细胞表面上FA受体的过表达而增强。在磁体的存在下观察到纳米缀合物和KB细胞之间的更强的相互作用。此外，激光和/或射频诱导的纳米缀合物与外部磁性应用的组合降低了KB细胞的活力。因此，本研究将为肿瘤多维治疗的发展开辟一个新的方向。(C)2015爱思唯尔有限公司版权所有。
We have developed multi-componential nanomaterials that are based on folic acid (FA) and polyethylenimine-functionalized carbon nanohorn conjugates that encapsulate magnetite nanoparticles. The nanoconjugates were characterized using a variety of experimental setups, including dynamic light scattering, thermogravimetric analysis, Fourier transform infrared spectrometry, and ultraviolet-visible-near-infrared (UV-Vis-NIR) spectroscopy. An NIR laser and a radio frequency-induction heating system were used to evaluate the heat-generating capability of the nanoconjugates. Aqueous solutions containing the nanoconjugates exhibited a significant increase in temperature after laser irradiation and/or radio frequency induction compared with controls. The intercellular distribution of the nanoconjugates was quantitatively analyzed using UV-Vis-NIR spectroscopy with two cell lines: human epidermal carcinoma (KB) and human embryonic (FHs173We) cells. The interaction between the FA-modified nanoconjugates and KB cells was enhanced through overexpression of FA receptors on the surface of the KB cells. A stronger interaction between the nanoconjugates and KB cells was observed in the presence of a magnet. Furthermore, laser-and/or radio frequency-induced nanoconjugates in combination with external magnetic application reduced the viability of KB cells. Therefore, the present study will open a new direction for the development of multi-dimensional cancer therapy. (C) 2015 Elsevier Ltd. All rights reserved.