Regulation of jasmonic acid biosynthesis by silicon application during physical injury to Oryza sativa L.
Regulation of jasmonic acid biosynthesis by silicon application during physical injury to Oryza sativa L.
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DOI：
10.1007/s10265-014-0641-3
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发表时间：
2014-07-01
影响因子：
2.8
通讯作者：
Lee, In-Jung
中科院分区：
文献类型：
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作者：
Kim, Yoon-Ha;Khan, Abdul Latif;Lee, In-Jung
研究了施硅对水稻植株的影响。以及其在创伤应激过程中对茉莉酸(JA)调节的反应。受害水稻植株内源茉莉酸含量显著高于非受害植株。相反，在创伤胁迫下，与非硅处理相比，硅处理显著减少了茉莉酸的合成。与未施硅和伤害胁迫相比，施硅和伤害胁迫下水稻植株脂肪氧合酶、烯氧合成酶1、烯氧合成酶2，12-氧代二烯酸还原酶3和丙二烯氧化环化酶基因的表达均下调。硅处理可调控生理伤害诱导的氧化胁迫，导致伤害胁迫下过氧化氢酶、过氧化物酶和多酚氧化酶活性高于未处理的植株。水稻体内较高的硅积累量也降低了膜脂过氧化水平，从而帮助水稻保护其免受伤害胁迫。综上所述，水稻植株中硅的积累通过抗氧化剂和茉莉酸的调节减轻了伤害的不利影响。
We investigated the effects of silicon (Si) application on rice plants (Oryza sativa L.) and its responses in the regulation of jasmonic acid (JA) during wounding stress. Endogenous JA was significantly higher in wounded rice plants than in non-wounded. In contrast, Si treatment significantly reduced JA synthesis as compared to non-Si applications under wounding stress. mRNA expression of O. sativa genes showed down-regulation of lipoxygenase, allene oxide synthase 1, allene oxide synthase 2, 12-oxophytodienoate reductase 3, and allene oxide cyclase upon Si application and wounding stress as compared to non-Si-treated wounded rice plants. The physical injury-induced-oxidative stress was modulated by Si treatments, which resulted in higher catalase, peroxidase, and polyphenol oxidase activities as compared with non-Si-treated plants under wounding stress. The higher Si accumulation in rice plants also reduced the level of lipid peroxidation, which helped the rice plants to protect it from wounding stress. In conclusion, Si accumulation in rice plants mitigated the adverse effects of wounding through regulation of antioxidants and JA.