Prevalent, protective, and convergent IgG recognition of SARS-CoV-2 non-RBD spike epitopes.
Prevalent, protective, and convergent IgG recognition of SARS-CoV-2 non-RBD spike epitopes.
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DOI：
10.1126/science.abg5268
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发表时间：
2021-06-04
期刊：
影响因子：
--
通讯作者：
Ippolito GC
中科院分区：
文献类型：
--
作者：
Voss WN;Hou YJ;Johnson NV;Delidakis G;Kim JE;Javanmardi K;Horton AP;Bartzoka F;Paresi CJ;Tanno Y;Chou CW;Abbasi SA;Pickens W;George K;Boutz DR;Towers DM;McDaniel JR;Billick D;Goike J;Rowe L;Batra D;Pohl J;Lee J;Gangappa S;Sambhara S;Gadush M;Wang N;Person MD;Iverson BL;Gollihar JD;Dye JM;Herbert AS;Finkelstein IJ;Baric RS;McLellan JS;Georgiou G;Lavinder JJ;Ippolito GC
SARS-CoV-2感染后在血浆中循环的免疫球蛋白G（IgG）抗体的分子组成和结合表位尚不清楚。在恢复期受试者中，IgG库对刺突糖蛋白的蛋白质组学去卷积揭示了应答主要（>80%）针对位于受体结合结构域（RBD）外部的表位。在一名受试者中，仅四种IgG谱系就占了93.5%的应答，其中包括一种针对N-末端结构域（NTD）的抗体，该抗体对致死性病毒攻击具有保护作用。对多供体类“公共”抗体的遗传、结构和功能表征揭示了NTD表位，该表位在新出现的SARS-CoV-2变异体中反复突变。这些数据表明，“公共”NTD导向和其他非RBD血浆抗体是普遍存在的，并对SARS-CoV-2保护和抗体逃逸有影响。
The molecular composition and binding epitopes of the immunoglobulin G (IgG) antibodies that circulate in blood plasma following SARS-CoV-2 infection are unknown. Proteomic deconvolution of the IgG repertoire to the spike glycoprotein in convalescent subjects revealed that the response is directed predominantly (>80%) against epitopes residing outside the receptor-binding domain (RBD). In one subject, just four IgG lineages accounted for 93.5% of the response, including an N-terminal domain (NTD)-directed antibody that was protective against lethal viral challenge. Genetic, structural, and functional characterization of a multi-donor class of “public” antibodies revealed an NTD epitope that is recurrently mutated among emerging SARS-CoV-2 variants of concern. These data show that “public” NTD-directed and other non-RBD plasma antibodies are prevalent and have implications for SARS-CoV-2 protection and antibody escape.
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影响因子：
64.5
作者：
Hou, Yixuan J.;Okuda, Kenichi;Baric, Ralph S.
通讯作者：
Baric, Ralph S.
影响因子：
14.9
作者：
Dunbar J;Krawczyk K;Leem J;Marks C;Nowak J;Regep C;Georges G;Kelm S;Popovic B;Deane CM
通讯作者：
Deane CM
影响因子：
56.9
作者：
Hsieh, Ching-Lin;Goldsmith, Jory A.;McLellan, Jason S.
通讯作者：
McLellan, Jason S.
DOI：
10.1107/s0907444904019158
发表时间：
2004-12-01
影响因子：
2.2
作者：
Emsley, P;Cowtan, K
通讯作者：
Cowtan, K
影响因子：
16.8
作者：
Henderson R;Edwards RJ;Mansouri K;Janowska K;Stalls V;Gobeil SMC;Kopp M;Li D;Parks R;Hsu AL;Borgnia MJ;Haynes BF;Acharya P
通讯作者：
Acharya P