The genome sequence of Mannheimia haemolytica A1:: Insights into virulence, natural competence, and Pasteurellaceae phylogeny
The genome sequence of Mannheimia haemolytica A1:: Insights into virulence, natural competence, and Pasteurellaceae phylogeny
复制标题
DOI：
10.1128/jb.00675-06
复制
发表时间：
2006-10-01
影响因子：
3.2
通讯作者：
Highlander, Sarah K.
中科院分区：
文献类型：
--
作者：
Gioia, Jason;Qin, Xiang;Highlander, Sarah K.
提出了牛呼吸道疾病综合症 (BRDC) 的病原体溶血性曼海姆菌的基因组序列草图。菌株 ATCC BAA-410 是从患有 BRDC 的小牛肺中分离出来的，是 DNA 来源。注释的基因组包括 2,839 个编码序列，其中 1,966 个被指定了功能，其中 436 个是溶血分枝杆菌独有的。通过基因组注释，确定了许多感兴趣的特征，包括噬菌体和与毒力、自然能力和转录调控相关的基因。除了之前描述的毒力因子外，溶血支原体还编码粘附素，包括丝状血凝素 FhaB 和两个三聚体自转运蛋白粘附素。还存在两种双功能免疫球蛋白蛋白酶/粘附素，以及第三种免疫球蛋白蛋白酶。与铁获取和耐药性相关的基因已被鉴定出来，并且可能对宿主的生存和毒力很重要。基因组分析表明，溶血分枝杆菌具有天然活性，因为存在天然活性基因和 DNA 摄取信号序列 (USS)。对巴氏杆菌科其他物种的能力位点和 USS 进行比较表明，溶血支原体、胸膜肺炎放线杆菌和杜克雷嗜血杆菌形成了与其他巴氏杆菌不同的谱系。这一观察结果得到了使用预测管家基因序列的系统发育分析的支持。
The draft genome sequence of Mannheimia haemolytica At, the causative agent of bovine respiratory disease complex (BRDC), is presented. Strain ATCC BAA-410, isolated from the lung of a calf with BRDC, was the DNA source. The annotated genome includes 2,839 coding sequences, 1,966 of which were assigned a function and 436 of which are unique to M. haemolytica. Through genome annotation many features of interest were identified, including bacteriophages and genes related to virulence, natural competence, and transcriptional regulation. In addition to previously described virulence factors, M. haemolytica encodes adhesins, including the filamentous hemagglutinin FhaB and two trimeric autotransporter adhesins. Two dual-function immunoglobulin-protease/adhesins are also present, as is a third immunoglobulin protease. Genes related to iron acquisition and drug resistance were identified and are likely important for survival in the host and virulence. Analysis of the genome indicates that M. haemolytica is naturally competent, as genes for natural competence and DNA uptake signal sequences (USS) are present. Comparison of competence loci and USS in other species in the family Pasteurellaceae indicates that M. haemolytica, Actinobacillus pleuropneumoniae, and Haemophilus ducreyi form a lineage distinct from other Pasteurellaceae. This observation was supported by a phylogenetic analysis using sequences of predicted housekeeping genes.