A High-Yield Synthesis of Deoxy-2-fluoroinosine and Its Incorporation into Oligonucleotides.
A High-Yield Synthesis of Deoxy-2-fluoroinosine and Its Incorporation into Oligonucleotides.
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脱氧-2-氟肌苷的高产合成及其掺入寡核苷酸。
DOI：
10.1002/chin.199732191
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发表时间：
1997
期刊：
影响因子：
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通讯作者：
J. Behr
中科院分区：
文献类型：
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作者：
A. Adib;P. Potier;S. Doronina;I. Huc;J. Behr
摘要描述了一种改进的脱氧-2-氟肌苷核苷合成方法，该方法利用温和的氟化（聚乙烯吡啶多氢氟化物）和O-甲硅烷基脱保护（三乙胺三氢氟化物）反应。衍生的5′-二甲氧基三苯甲基-2-氟肌苷-3 ′-亚磷酰胺被掺入到含有多达7个非天然碱基的10-、15-和20- mer寡核苷酸中。© 1997由Elsevier Science Ltd.出版。
Abstract An improved synthesis of the deoxy-2-fluoroinosine nucleoside is described, that makes use of mild fluorination (polyvinylpyridinium polyhydrogenfluoride) and O-silyl deprotection (triethylamine trihydrofluoride) reactions. The derived 5′-dimethoxytrityl-2-fluoroinosine-3′-phosphoramidite was incorporated into 10-, 15- and 20- mer oligonucleotides containing up to 7 non-natural bases. © 1997 Published by Elsevier Science Ltd.