A new method for the determination of carboxyhemoglobin in blood of newborns. Possible clinical implications of elevated levels.
A new method for the determination of carboxyhemoglobin in blood of newborns. Possible clinical implications of elevated levels.
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测定新生儿血液中碳氧血红蛋白的新方法
DOI：
10.3109/00365519009087519
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发表时间：
1990
期刊：
影响因子：
--
通讯作者：
W. Heerspink
中科院分区：
文献类型：
--
作者：
A. Zwart;H. Siepel;W. Heerspink
由于成人血红蛋白（HbA）和胎儿血红蛋白（HbF）的光谱差异，使得新生儿血液中碳氧血红蛋白（COHb）的准确测定变得困难。只有最新的COHb测定专用仪器才考虑到这些差异。然而，由于在脱氧状态下两种血红蛋白之间几乎没有光谱差异，用连二亚硫酸钠还原新生儿血液消除了分光光度法的误差。基于这一原理，开发了一种简单，快速和准确的方法来测定微量血液中的COHb;同样适用于新生儿和成人的血液。与该新方法一起使用的仪器是通用离心临床化学分析仪（COBAS-FARA，Roche，Basle，Switzerland）。在用连二亚硫酸钠还原血液之后，形成简单的双组分系统（Hb和COHb），其可以通过在两个合适的波长下测量来进行比色定量，即，579 nm和534 nm。在以下公式的帮助下计算COHb分数：COHb % = 177.73。(A534/A579）-176.66。与OSM 3血氧仪（Radiometer A/S，丹麦）比较，145份具有不同COHb水平（0-30%）的成人血液样本的COHb %平均差异约为0.06%（SD = 0.38）。对于胎儿血液（N = 63），当在所谓的胎儿模式中使用OSM 3时，发现大约相同的差异，而与常规成人模式的比较产生大约3.1%的差异。这表明新方法确实不受HbA和HbF之间的光谱差异的干扰。（250字处删节）
Spectral differences between hemoglobin derivatives from adult-(HbA) and fetal-hemoglobin (HbF) make the accurate determination of carboxyhemoglobin (COHb) troublesome in blood of newborns. Only the newest dedicated instruments for the determination of COHb take these differences into account. However, since there are virtually no spectral differences between both hemoglobins in the deoxygenated state, reduction of neonatal blood with sodium dithionite eliminates that spectrophotometric error. Based on this principle, an easy, fast and accurate method for the determination of COHb in minimal amounts of blood was developed; equally well suited for blood of newborns and adults. The instrument used with this new method is a general purpose centrifugal clinical chemistry analyzer (COBAS-FARA, Roche, Basle, Switzerland). After the reduction of blood with sodium dithionite, a simple two component system (Hb and COHb) is formed, which can be spectrophotometrically quantitated by measuring at two suitable wavelengths, i.e., 579 nm and 534 nm. The COHb fraction is calculated with the help of: COHb % = 177.73.(A534/A579)-176.66. Comparison with the OSM3 Hemoximeter (Radiometer A/S, Denmark) for 145 adult blood samples with varying COHb levels (0-30%) yielded a mean difference in COHb % of about 0.06% (SD = 0.38). For fetal blood (N = 63) about the same difference was found when the OSM3 was used in the so-called fetal mode, while comparison with the regular adult mode yielded a difference of about 3.1%. This indicates that the new method is indeed not disturbed by the spectral differences between HbA and HbF.(ABSTRACT TRUNCATED AT 250 WORDS)
足月儿和早产儿血液碳氧血红蛋白浓度和一氧化碳排泄率的配对测定。
DOI：
--
发表时间：
1982
期刊：
The Journal of laboratory and clinical medicine
影响因子：
--
作者：
Ostrander,CR;Cohen,RS;Hopper,AO;Cowan,BE;Stevens,GB;Stevenson,DK
通讯作者：
Stevenson,DK