Essential Role of DPPA3 for Chromatin Condensation in Mouse Oocytogenesis
Essential Role of DPPA3 for Chromatin Condensation in Mouse Oocytogenesis
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DOI：
10.1095/biolreprod.111.095018
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发表时间：
2012-02-01
影响因子：
3.6
通讯作者：
Nakano, Toru
中科院分区：
文献类型：
--
作者：
Liu, Yu-Jung;Nakamura, Toshinobu;Nakano, Toru
哺乳动物卵母细胞发生过程中染色质构型发生动态变化。根据染色质构型模式，完全发育的卵母细胞分为两种类型。一种是围绕核仁（SN）型卵母细胞，另一种是非围绕核仁（NSN）型卵母细胞。虽然在从NSN型卵母细胞到SN型卵母细胞的转变过程中染色质凝聚是正常早期胚胎发育的先决条件，但其分子机制仍不清楚。在这项研究中，我们分析了DPPA 3（也称为PGC 7/Stella）在这种转变中的作用，使用Dppa 3-null卵母细胞。NSN到SN的转换显着受损，转录抑制是不完整的Dppa 3-null卵母细胞。此外，我们发现，事先转录抑制是必要的NSN到SN的过渡。这些发现表明DPPA 3是通过转录抑制和染色质凝聚产生功能性卵母细胞的重要因子。
Dynamic alterations in chromatin configuration occur in mammalian oocytogenesis. Based on chromatin configuration patterns, fully grown oocytes are classified into two types. One is surrounded nucleolus (SN)-type and the other is nonsurrounded nucleolus (NSN)-type oocytes. Although chromatin condensation during the transition from NSN- to SN-type oocytes is a prerequisite for normal early embryonic development, the molecular mechanisms remain unclear. In this study, we analyzed the role of DPPA3 (also known as PGC7/Stella) in this transition using Dppa3-null oocytes. The NSN-to-SN transition was significantly impaired, and transcriptional repression was incomplete in the Dppa3-null oocytes. Additionally, we revealed that prior transcriptional repression was necessary for the NSN-to-SN transition. These findings demonstrate that DPPA3 is an essential factor for the production of functional oocytes through transcriptional repression and chromatin condensation.