Chromatin structure and analysis of mechanisms of activators and repressors.
Chromatin structure and analysis of mechanisms of activators and repressors.
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DOI：
10.1006/meth.1998.0632
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发表时间：
1998-08
期刊：
影响因子：
4.8
通讯作者：
R. Simpson
中科院分区：
文献类型：
--
作者：
R. Simpson
一些转录抑制因子似乎至少是其机制的一部分来组织染色质结构。一些转录激活因子似乎至少部分地改变或改造了染色质结构。因此，理解转录调控需要研究不同功能活性状态下特定基因的染色质结构的方法。本文综述了测定染色质结构的化学和酶的方法，用于分析结果的方法，以及解释数据推断染色质结构的标准。
Some transcriptional repressors appear to organize chromatin structure as at least part of their mechanism. Some transcriptional activators appear to alter or remodel chromatin structure as at least part of their mechanism. Understanding transcriptional regulation thus requires methods for investigation of the chromatin structure of specific genes in different states of functional activity. This paper reviews chemical and enzymatic approaches to determination of chromatin structure, the methods used for analysis of the results, and criteria for interpretation of the data to infer chromatin structures.