Identification of host-immune response protein candidates in the sera of human oral squamous cell carcinoma patients.
Identification of host-immune response protein candidates in the sera of human oral squamous cell carcinoma patients.
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DOI：
10.1371/journal.pone.0109012
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发表时间：
2014
期刊：
影响因子：
3.7
通讯作者：
Gopinath SC
中科院分区：
文献类型：
--
作者：
Chen Y;Azman SN;Kerishnan JP;Zain RB;Chen YN;Wong YL;Gopinath SC
口腔鳞状细胞癌（OSCC）是世界上最常见的癌症之一，它与显著的死亡率相关，并与几个风险因素有关。值得注意的是，未能在早期发现这些肿瘤是提高口腔鳞癌患者生存率和生活质量的根本障碍。在本研究中，OSCC患者（n = 25）和健康对照（n = 25）的血清样本进行双向凝胶电泳（2-DE）和银染色，以确定生物标志物，可能允许早期诊断。 在这方面，通过高分辨率MALDI-TOF质谱法对对应于各种上调和下调蛋白质的2-DE点进行测序，并使用MASCOT数据库进行分析。我们在口腔鳞癌患者血清中鉴定了以下差异表达的宿主特异性蛋白：富含亮氨酸的α2-糖蛋白（LRG）、α-1-B-糖蛋白（ABG）、丛生蛋白（CLU）、PRO2044、触珠蛋白（HAP）、补体C3（C3）、前载脂蛋白A1（proapo-A1）和视黄醇结合蛋白4前体（RBP 4）。此外，还检测到5种非宿主因子，包括来自鲁氏不动杆菌、多噬伯克霍尔德氏菌、黄色粘球菌、香港落叶松杆菌和唾液链球菌的细菌抗原。随后，我们分析了这些蛋白质的免疫原性，使用合并血清从口腔鳞癌患者。在这方面，发现这些候选生物标志物中的五个是免疫反应性的：CLU、HAP、C3、proapo-A1和RBP 4。总之，我们的免疫蛋白质组学方法已经确定了各种血清生物标志物，可以促进口腔鳞癌早期诊断工具的发展。
One of the most common cancers worldwide is oral squamous cell carcinoma (OSCC), which is associated with a significant death rate and has been linked to several risk factors. Notably, failure to detect these neoplasms at an early stage represents a fundamental barrier to improving the survival and quality of life of OSCC patients. In the present study, serum samples from OSCC patients (n = 25) and healthy controls (n = 25) were subjected to two-dimensional gel electrophoresis (2-DE) and silver staining in order to identify biomarkers that might allow early diagnosis. In this regard, 2-DE spots corresponding to various up- and down-regulated proteins were sequenced via high-resolution MALDI-TOF mass spectrometry and analyzed using the MASCOT database. We identified the following differentially expressed host-specific proteins within sera from OSCC patients: leucine-rich α2-glycoprotein (LRG), alpha-1-B-glycoprotein (ABG), clusterin (CLU), PRO2044, haptoglobin (HAP), complement C3c (C3), proapolipoprotein A1 (proapo-A1), and retinol-binding protein 4 precursor (RBP4). Moreover, five non-host factors were detected, including bacterial antigens from Acinetobacter lwoffii, Burkholderia multivorans, Myxococcus xanthus, Laribacter hongkongensis, and Streptococcus salivarius. Subsequently, we analyzed the immunogenicity of these proteins using pooled sera from OSCC patients. In this regard, five of these candidate biomarkers were found to be immunoreactive: CLU, HAP, C3, proapo-A1 and RBP4. Taken together, our immunoproteomics approach has identified various serum biomarkers that could facilitate the development of early diagnostic tools for OSCC.
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