The significance of serum xanthine oxidase and oxidation markers in acute paraquat poisoning in humans.
The significance of serum xanthine oxidase and oxidation markers in acute paraquat poisoning in humans.
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DOI：
10.1016/j.clinbiochem.2010.09.006
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发表时间：
2011-02
影响因子：
2.8
通讯作者：
Juanwen Zhang;G. Lv;Ying Zhao
中科院分区：
文献类型：
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作者：
Juanwen Zhang;G. Lv;Ying Zhao
目的测定人类急性百草枯中毒中的黄嘌呤氧化酶 (XO) 活性和氧化应激参数。设计和方法招募了 55 名患者和 26 名健康对照参与者。采用分光光度法测定血清XO、超氧化物歧化酶(SOD)、谷胱甘肽(GSH)和丙二醛(MDA)。结果患者组XO活性和MDA水平较高,SOD活性和GSH水平较低。百草枯中毒死亡组XO活性和MDA水平显着升高，GSH水平和SOD活性降低。患者组XO活性与SOD、GSH均呈显着负相关，而与MDA水平呈显着正相关。结论XO活性有助于百草枯中毒患者氧化损伤的发生。血清XO活性可作为人类急性百草枯中毒预后的标志物。
OBJECTIVESDetermine xanthine oxidase (XO) activity and oxidative-stress parameters in human acute paraquat poisoning.DESIGN AND METHODSTwnty-five patients and 26 healthy control participants were recruited. Serum XO, superoxide dismutase (SOD), Glutathione (GSH) and malondialdehyde (MDA) were determined by spectrophotometric methods.RESULTSXO activity and MDA levels were higher and SOD activity and GSH level were lower in the patients group. XO activity and MDA level were significantly higher, and the GSH level and SOD activity were lower in the paraquat poisoning fatality group. There was a significant negative correlation between the XO activity and both the SOD and GSH, while there was a significant positive correlation between XO activity and the MDA level in the patients group.CONCLUSIONSXO activity contributes to the development of oxidative injury in patients with paraquat poisoning. Serum XO activity could be used as a marker for prognosis in human acute paraquat intoxication.