11β-Hydroxysteroid dehydrogenase complementary deoxyribonucleic acid in rainbow trout:: Cloning, sites of expression, and seasonal changes in gonads
11β-Hydroxysteroid dehydrogenase complementary deoxyribonucleic acid in rainbow trout:: Cloning, sites of expression, and seasonal changes in gonads
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DOI：
10.1210/en.2002-220446
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发表时间：
2003-06-01
期刊：
影响因子：
4.8
通讯作者：
Young, G
中科院分区：
文献类型：
--
作者：
Kusakabe, M;Nakamura, I;Young, G
11 β-羟基类固醇脱氢酶（11beta-Hydroxysteroids，11beta-HSDs）是哺乳动物体内重要的类固醇合成酶，催化活性糖皮质激素（皮质醇和皮质酮）和惰性11-酮形式（可的松和11-脱氢皮质酮）的相互转化。在硬骨鱼中，11 β-HSD也在雄鱼中主要雄激素11-酮睾酮的产生中发挥作用。在这项研究中，我们克隆了编码虹鳟鱼11 β-HSD（rt 11 β-HSD）的cDNA从睾丸和头肾。预测的氨基酸序列、疏水性分析和在HEK 293细胞中使用rt 11 β-HSD的瞬时转染试验表明，rt 11 β-HSD是哺乳动物11 β-HSD 2型的同源物。rt 11 β-HSD转录物存在于类固醇生成组织和许多其它组织中。在睾丸间质细胞，早期卵黄形成卵泡的卵泡膜细胞，中期卵黄形成卵泡和排卵后卵泡的卵泡膜和颗粒细胞中发现了rt 11 β-HSD转录物的强原位杂交信号。较弱的信号也被发现在头肾肾间细胞从少年虹鳟鱼。睾丸中rt 11 β-HSD转录物的季节性变化显示出与应激诱导的血清皮质醇水平相似的模式，但与血清雄激素水平无关。高水平的rt 11 β-HSD转录本被发现在卵巢卵泡从晚期卵黄通过排卵。这些结果提高了rt 11 β-HSD在保护发育中的性腺免受应激诱导的皮质醇的抑制作用中的作用的可能性。
11beta-Hydroxysteroid dehydrogenases (11beta-HSDs) are important steroidogenic enzymes for catalyzing the interconversion of active glucocorticoid (cortisol and corticosterone) and inert 11-keto forms (cortisone and 11-dehydrocorticosterone) in mammals. In teleosts, 11beta-HSD also plays a role in the production of the predominant androgen, 11-ketotestosterone, in male fish. In this study we cloned cDNAs encoding rainbow trout 11beta-HSD (rt11beta-HSD) from testes and head kidney. The predicted amino acid sequence, hydrophobicity analysis, and transient transfection assays with rt11beta-HSD in HEK293 cells showed that rt11beta-HSD is a homolog of mammalian 11beta-HSD type 2. rt11beta-HSD transcripts are present in steroidogenic tissues and in a number of other tissues. Strong in situ hybridization signals for rt11beta-HSD transcripts were found in Leydig cells of testes, in thecal cells of the early vitellogenic ovarian follicles, and in thecal and granulosa cells of the mid-vitellogenic and postovulatory follicles. Weaker signals were also found in head kidney interrenal cells from juvenile rainbow trout. Seasonal changes in rt11beta-HSD transcripts in testes showed a pattern similar to that of stress-induced serum cortisol levels, but not to serum androgen levels. High levels of rt11beta-HSD transcripts were found in ovarian follicles from late vitellogenesis through ovulation. These results raise the possibility of a role for rt11beta-HSD in the protection of developing gonads from the inhibitory effects of stress-induced cortisol.