THYMIC REQUIREMENT FOR CLONAL DELETION DURING T-CELL DEVELOPMENT
THYMIC REQUIREMENT FOR CLONAL DELETION DURING T-CELL DEVELOPMENT
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DOI：
10.1126/science.2511630
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发表时间：
1989-11-24
期刊：
影响因子：
56.9
通讯作者：
MATIS, LA
中科院分区：
文献类型：
--
作者：
FRY, AM;JONES, LA;MATIS, LA
在T细胞分化过程中，自身耐受性部分通过胸腺内自身反应性T细胞的缺失（阴性选择）来建立。T细胞受体（TCR）-α的存在。β +在老年无胸腺（nu/nu）小鼠中的T细胞表明一些T细胞也可以在没有胸腺影响的情况下成熟。因此，为了确定胸腺是否是阴性选择所必需的，TCR V β在无胸腺nu/nu小鼠和它们的同类正常同窝仔中比较表达。表达V β 3蛋白的T细胞对次要淋巴细胞刺激（Mlsc）决定簇具有特异性，并且由于Mlsc+小鼠品系中的自身耐受性而在胸腺内缺失。这里显示在Mlsc+ BALB/nu/+小鼠中V β 3 + T细胞缺失，但在其BALB/c nu/nu同窝出生的小鼠中没有。因此，T细胞发育期间克隆缺失需要胸腺。
During T cell differentiation, self tolerance is established in part by the deletion of self-reactive T cells within the thymus (negative selection). The presence of T cell receptor (TCR)-.alpha..beta.+ cells in older athymic (nu/nu) mice indicates that some T cells can also mature without thymic influence. Therefore, to determine whether the thymus is required for negative selection, TCR V.beta. expression was compared in athymic nu/nu mice and their congenic normal littermates. T cells expressing V.beta.3 proteins are specific for minor lymphocyte stimulatory (Mlsc) determinants and are deleted intrathymically due to self tolerance in Mlsc+ mouse strains. Here it is shown that V.beta.3+ T cells are deleted in Mlsc+ BALB/nu/+ mice, but not in their BALB/c nu/nu littermates. Thus, the thymus is required for clonal deletion during T cell development.