Fluorescence resonance energy transfer studies of aminoglycoside binding to a T box antiterminator RNA
Fluorescence resonance energy transfer studies of aminoglycoside binding to a T box antiterminator RNA
复制标题
DOI：
10.1016/j.bmcl.2005.02.007
复制
发表时间：
2005-04-15
影响因子：
2.7
通讯作者：
Hines, JV
中科院分区：
文献类型：
--
作者：
Means, JA;Hines, JV
The T box transcription antitermination mechanism is found in many Gram-positive bacteria. The T box genes are typically tRNA synthetase, amino acid biosynthesis, and amino acid transport genes that have a common transcriptional control mechanism in which a unique RNA-RNA interaction occurs between an uncharged tRNA and the 5 ' leader region of the nascent mRNA, leading to antitermination of transcription. The tRNA binds the mRNA in at least two regions: the specifier sequence and the antiterminator. If the latter interaction does not occur, then transcription is terminated. The binding of eight different aminoglycosides to a model of the Bacillus subtilis tyrS T box antiterminator RNA has been studied using fluorescence resonance energy transfer. The observed single-site binding dissociation constants were in the low to mid micromolar range. The structure-activity relationship of aminoglycoside binding indicates that selective binding of small molecules to T box antiterminator RNA can be achieved. (c) 2005 Elsevier Ltd. All rights reserved.
