Directed Assembly of DNA-Functionalized Gold Nanoparticles Using Pyrrole-Imidazole Polyamides
Directed Assembly of DNA-Functionalized Gold Nanoparticles Using Pyrrole-Imidazole Polyamides
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DOI：
10.1021/ja3014924
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发表时间：
2012-05-23
影响因子：
15
通讯作者：
Graham, Duncan
中科院分区：
文献类型：
--
作者：
Krpetic, Zeljka;Singh, Ishwar;Graham, Duncan
构建纳米粒子阵列和晶格的传统方法利用单链DNA序列的沃森-克里克碱基配对作为自组装的代理。尽管这种方法已经在纳米组装、诊断和生物医学等领域得到了广泛应用，但通过开发识别双链DNA （dsDNA）序列碱基对的策略，这种识别词汇的多样性可以大大增加。本文首次报道了利用吡咯-咪唑聚酰胺-GNP （PA-GNP）偶联物识别dsDNA序列的程序化金纳米粒子（GNP）聚集。我们证明了在完全匹配的dsDNA序列相对于包含一个和两个碱基对不匹配的dsDNA序列存在时形成GNP聚合体的这种策略的可逆性和选择性。
Traditional methods for the construction of nanoparticle arrays and lattices exploit Watson-Crick base pairing of single-stranded DNA sequences as a proxy for self-assembly. Although this approach has been utilized in a variety of applications in nanoassembly, diagnostics, and biomedicine, the diversity of this recognition lexicon, could be considerably increased by developing strategies that recognize the base-pairing landscape of double-stranded DNA (dsDNA) sequences. Herein we describe the first report of programmed gold nanoparticle (GNP) aggregation directed by the recognition of dsDNA sequences using pyrrole-imidazole polyamide-GNP (PA-GNP) conjugates. We demonstrate the reversibility and selectivity of this strategy for forming GNP aggregates in the presence of fully matched dsDNA sequences relative to dsDNA sequences containing one- and two-base-pair mismatches.