Pollen sporopollenin:: degradation and structural elucidation
Pollen sporopollenin:: degradation and structural elucidation
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DOI：
10.1007/s004970050189
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发表时间：
1999-09-01
影响因子：
--
通讯作者：
Heredia, A
中科院分区：
文献类型：
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作者：
Domínguez, E;Mercado, JA;Heredia, A
我们报道了从不同物种的花粉中分离纯化的孢粉蛋白及其完全增溶作用。以氟化氢为萃取剂，在吡啶中提取了赤松、白桦、豚草和辣椒的外皮。这些外壁是通过酸解和皂化处理从它们的芳香部分和通过酯键连接的脂肪酸中提纯出来的。得到的生物聚合物几乎完全保持了原始花粉颗粒的形状。傅里叶变换红外光谱分析表明，分离得到的孢粉素中不含多糖和酚类物质，存在不饱和羧酸基和醚键。在室温下，通过24小时的穷尽臭氧降解成功地降解了孢粉。温和的臭氧氧化(在0摄氏度下3小时)并不能完全降解生物聚合物。用气相色谱-质谱联用法对臭氧化后的化合物进行了分析。鉴定出三种植物中的主要成分是碳原子数较低的二元酸，分别占总化合物的28.8%、63.2%和88.5%。在松材线虫、松材线虫和白僵菌孢粉中也发现了脂肪酸和正构烷烃。根据所获得的数据，提出了关于孢粉蛋白的化学性质和结构排列的假设。
We report the isolation of purified sporopollenin from pollen grains of different species and its complete solubilization. Exine from Pinus pinaster, Betula alba, Ambrosia elatior and Capsicum annuum was extracted by treatment with hydrogen fluoride in pyridine. These exines were purified from their aromatic moieties and from fatty acids linked by ester bonds using acidolysis and saponification treatments. The biopolymer obtained retains almost completely the shape of the original pollen grain. Fourier-transform infrared spectroscopy analysis of the isolated sporopollenin showed the absence of polysaccharide and phenolic material and the presence of carboxylic acid groups joined to unsaturations and ether linkages. Sporopollenin samples were successfully degraded by exhaustive 24-h ozonolysis at room temperature. Gentle ozonolysis (3 h at 0 degrees C) did not completely degrade the biopolymer. The compounds obtained after exhaustive ozonolysis were analysed by gas chromatography-mass spectrometry. Dicarboxylic acids with a low number of carbon atoms were identified as major components of sporopollenin from P. pinaster, A. elatior and C. annuum, representing 28.8%, 63.2% and 88.5%, respectively, of the total compounds obtained. Fatty acids and n-alkanes also were identified in P. pinaster, A. elatior and B. alba sporopollenin. From the data obtained, an hypothesis about the chemical nature and structural arrangement of the sporopollenin is proposed.