Kinetics, Affinity, Thermodynamics, and Selectivity of Phosphate Removal Using Immobilized Phosphate-Binding Proteins
Kinetics, Affinity, Thermodynamics, and Selectivity of Phosphate Removal Using Immobilized Phosphate-Binding Proteins
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使用固定化磷酸盐结合蛋白去除磷酸盐的动力学、亲和力、热力学和选择性
DOI：
10.1021/acs.est.0c02272
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发表时间：
2020
影响因子：
11.4
通讯作者：
Mayer, Brooke K.
中科院分区：
文献类型：
--
作者：
Venkiteshwaran, Kaushik;Wells, Erin;Mayer, Brooke K.
磷酸盐（Pi）的选择性吸附系统具有固定化的Pi结合蛋白（PBP），最近引起了人们的注意，超低Pi去除，然后恢复。研究了固定化PBP（PBP树脂）对P1吸附的动力学、亲和性、热力学和选择性，以及pH和温度对P1吸附的影响。固定化PBP不影响其Piaffinity。在22 °C和pH7.1条件下的动力学研究表明，PBP树脂在0.64 ± 0.2 min内达到平衡容量的95%。估算的Langmuir亲和常数（KL）为21 ± 5 μM-1 Pi（220 ± 52 L/mg-Pi），高于最近文献报道的Pi吸附剂。使用PBP树脂的高亲和力Piadsorption的理想操作范围为pH 4.5至9和温度14至37 °C。常见阴离子（Cl-、Br-、NO2-、NO3-、SO 42-和HCO 3-）的存在对Pi-PBP树脂吸附过程没有影响。使用Pi-PBP树脂的吸附是放热的（ΔH= −6.3 ± 1.3 kJ/mol）和自发的（ΔG= −39.7 ± 0.1至−43.2 ± 0.2 kJ/mol）在14和43 °C之间。结果表明，PBP树脂的吸附速率和亲和力均优于现有的吸附剂。今后的工作，以提高PBP树脂的吸附容量是重要的，它的应用作为一个可行的Piadsorbent。
A phosphate (Pi)-selective adsorption system featuring immobilized Pi-binding proteins (PBP) has recently attracted attention for ultralow Piremoval followed by recovery. This study investigated the adsorption kinetics, affinity, thermodynamics, and selectivity, as well as the effect of pH and temperature on Piadsorption using immobilized PBP (PBP resin). Immobilizing PBP did not affect its Piaffinity. Kinetic studies at 22 °C and pH 7.1 showed that the PBP resin achieved 95% of its equilibrium capacity within 0.64 ± 0.2 min. The estimated Langmuir affinity constant (KL) was 21 ± 5 μM–1Pi(220 ± 52 L/mg-Pi), which is higher than Piadsorbents recently reported in literature. The ideal operating ranges for high-affinity Piadsorption using PBP resin were pH 4.5 to 9 and temperature 14 to 37 °C. The Pi-PBP resin adsorption process was not affected by the presence of common anions (Cl–, Br–, NO2–, NO3–, SO42–, and HCO3–). Adsorption using the Pi-PBP resin was exothermic (ΔH= −6.3 ± 1.3 kJ/mol) and spontaneous (ΔG= −39.7 ± 0.1 to −43.2 ± 0.2 kJ/mol) between 14 and 43 °C. These results indicate that PBP resin’s Piadsorption rate and affinity surpass those of existing adsorbents. Future work to increase the PBP resin’s adsorption capacity is important to its application as a viable Piadsorbent.