The accumulation of β-aminobutyric acid is controlled by the plant's immune system
The accumulation of β-aminobutyric acid is controlled by the plant's immune system
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DOI：
10.1007/s00425-017-2751-3
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发表时间：
2017-10-01
期刊：
影响因子：
4.3
通讯作者：
Mauch-Mani, Brigitte
中科院分区：
文献类型：
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作者：
Baccelli, Ivan;Glauser, Gaetan;Mauch-Mani, Brigitte
在分子识别病原体存在后，内源β-氨基丁酸(BABA)水平升高。BABA在不同的抗病或感病过程中积累不同。最近发现的启动分子β-氨基丁酸是植物的天然产物，这对先前发现拟南芥的感知机制具有重要意义。研究发现，在非生物胁迫或感染强毒病原体后，BABA水平升高，但内源BABA在防御中的作用仍有待确定。为了研究植物-病原菌相互作用过程中内源BABA变化的生物学意义，我们研究了毒力、无毒(AvrRpt2)和非致病(HrpA)菌株如何感染丁香假单胞菌。番茄DC3000(PST DC3000)以及防御激发子(Flg22和AtPep2)处理影响BABA在拟南芥中的积累。我们发现，在对PST DC3000的抗性过程中，BABA水平的上升速度比对PST DC3000敏感期间更快。此外，在感染非致病性PST DC3000 hrpA突变体或用激发子处理后，BABA在PAMP触发免疫(PTI)过程中积累。重要的是，Flg22处理在Columbia-0植物中诱导了Baba的上升，但在Wassilewski ja-0植物中却没有，因为Wassilewski ja-0植物本身就拥有一个不起作用的鞭毛受体。这些结果表明，BABA的水平受植物免疫系统的控制，从而促进了对植物产生BABA的生物学作用的理解。
Endogenous levels of beta-aminobutyric acid (BABA) increase after the molecular recognition of pathogen presence. BABA is accumulated differently during resistance or susceptibility to disease.The priming molecule beta-aminobutyric acid has been recently shown to be a natural product of plants, and this has provided significance to the previous discovery of a perception mechanism in Arabidopsis. BABA levels were found to increase after abiotic stress or infection with virulent pathogens, but the role of endogenous BABA in defence has remained to be established. To investigate the biological significance of endogenous BABA variations during plant-pathogen interactions, we investigated how infections with virulent, avirulent (AvrRpt2), and non-pathogenic (hrpA) strains of Pseudomonas syringae pv. tomato DC3000 (Pst DC3000), as well as treatment with defence elicitors (Flg22 and AtPep2), affect the accumulation of BABA in Arabidopsis plants. We found that BABA levels increased more rapidly during resistance than susceptibility to Pst DC3000. In addition, BABA was accumulated during PAMP-triggered immunity (PTI) after infection with the non-pathogenic Pst DC3000 hrpA mutant, or treatment with elicitors. Importantly, treatment with Flg22 induced BABA rise in Columbia-0 plants but not in Wassilewskija-0 plants, which naturally possess a non-functional flagellin receptor. These results indicate that BABA levels are controlled by the plant's immune system, thus advancing the understanding of the biological role of plant produced BABA.