Sum1 and Ndt80 proteins compete for binding to middle sporulation element sequences that control meiotic gene expression
Sum1 and Ndt80 proteins compete for binding to middle sporulation element sequences that control meiotic gene expression
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DOI：
10.1128/mcb.23.14.4814-4825.2003
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发表时间：
2003-07-01
影响因子：
5.3
通讯作者：
Vershon, AK
中科院分区：
文献类型：
--
作者：
Pierce, M;Benjamin, KR;Vershon, AK
减数分裂中的一个关键转变是从前期退出并进入核分裂，这在酿酒酵母中取决于中间孢子形成基因的诱导。Ndt 80是中间孢子形成基因的主要转录激活因子，并与称为中间孢子形成元件（MSE）的DNA序列元件结合。Sum 1是一种转录抑制因子，在有丝分裂和早期孢子形成过程中与MSE结合并抑制中间孢子形成基因。我们证明，Smut和Ndt 80具有重叠的，但不同的序列要求，结合和作用于变异MSE。全基因组表达分析确定了一个亚组的中间孢子形成基因，是去阻遏的总和1突变体。比较Sum 1-阻遏型启动子中的MSE和来自其他中间孢子形成基因的MSE，发现存在不同类别的MSE。我们发现，Sum 1和Ndt 80竞争结合到MSE和MSE的序列中的小的变化可以产生很大的差异，其中蛋白质结合。我们的研究结果提供了一个机制，通过Sum 1阻遏物和Ndt 80激活剂之间的竞争差异调节中间孢子形成基因的表达。
A key transition in meiosis is the exit from prophase and entry into the nuclear divisions, which in the yeast Saccharomyces cerevisiae depends upon induction of the middle sporulation genes. Ndt80 is the primary transcriptional activator of the middle sporulation genes and binds to a DNA sequence element termed the middle sporulation element (MSE). Sum1 is a transcriptional repressor that binds to MSEs and represses middle sporulation genes during mitosis and early sporulation. We demonstrate that Smut and Ndt80 have overlapping yet distinct sequence requirements for binding to and acting at variant MSEs. Whole-genome expression analysis identified a subset of middle sporulation genes that was derepressed in a sum1 mutant. A comparison of the MSEs in the Sum1-repressible promoters and MSEs from other middle sporulation genes revealed that there are distinct classes of MSEs. We show that Sum1 and Ndt80 compete for binding to MSEs and that small changes in the sequence of an MSE can yield large differences in which protein is bound. Our results provide a mechanism for differentially regulating the expression of middle sporulation genes through the competition between the Sum1 repressor and the Ndt80 activator.