Evidence for neurotransmitter plasticity in vivo: developmental changes in properties of cholinergic sympathetic neurons.
Evidence for neurotransmitter plasticity in vivo: developmental changes in properties of cholinergic sympathetic neurons.
复制标题
体内神经递质可塑性的证据：胆碱能交感神经元特性的发育变化。
DOI：
10.1016/0012-1606(83)90365-2
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发表时间：
1983
影响因子：
2.7
通讯作者：
Keefe,D
中科院分区：
文献类型：
--
作者：
Landis,SC;Keefe,D
我们研究了成年和发育中大鼠足垫汗腺的胆碱能交感神经支配。乙酰胆碱酯酶染色显示成年大鼠的汗腺中有一丛重度染色的纤维。反应产物出现在离分泌小管细胞相当远的轴突束之间和周围。每束包含8-12个轴突，其具有许多静脉曲张，并包含小的透明和大的致密核心囊泡。后爪的腺体及其神经支配在出生后的前三周内发育。含儿茶酚胺的轴突与腺体的形成有关。在7和10天，强烈的荧光纤维包围的小管，和所有的轴突配置文件与腺体含有小颗粒囊泡（SGV）高锰酸盐固定后，揭示囊泡存储的去甲肾上腺素。在14天的汗腺丛是不那么强烈的荧光比在早期的年龄和相对较少的SGV。到第21天，没有内源性儿茶酚胺荧光和SGV检测。然而，暴露于外源性儿茶酚胺后，荧光纤维存在于成熟大鼠的汗腺中，它们在位置和密度上与乙酰胆碱酯酶染色定位的神经丛相对应。在寒冷和desmethylimipramine孵育阻断儿茶酚胺摄取，并没有观察到在虹膜或唾液腺的胆碱能副交感神经纤维。腹腔注射5-羟基多巴胺和高锰酸盐固定后，汗腺中的所有轴突都含有少量SGV。因此，发育中的汗腺似乎是由去甲肾上腺素能轴突支配的，去甲肾上腺素能轴突在成熟的腺体中精心制作轴突丛时失去了其内源性儿茶酚胺的储存，但没有失去其摄取和储存的能力。这些观察结果支持了这一假设，即胆碱能交感神经元似乎经历了从去甲肾上腺素能到胆碱能功能的转变，在体内发育过程中，类似于先前在细胞培养中所描述的。
We have examined the cholinergic sympathetic innervation of sweat glands in footpads of adult and developing rats. Acetylcholinesterase staining reveals a plexus of heavily stained fibers in the sweat glands of adult rats. Reaction product appears among and around bundles of axons that lie at a considerable distance from the cells of the secretory tubule. Each bundle contains 8–12 axons that possess numerous varicosities and contain small clear and large dense core vesicles. The glands of the hindpaws and their innervation develop during the first three weeks after birth. Catecholamine-containing axons were associated with the forming glands. At 7 and 10 days, intensely fluorescent fibers surrounded the tubules, and all of the axon profiles associated with the glands contained small granular vesicles (SGV) after permanganate fixation to reveal vesicular stores of norepinephrine. At 14 days the sweat gland plexus was less intensely fluorescent than at earlier ages and relatively few SGV were present. By 21 days, no endogenous catecholamine fluorescence and no SGV were detectable. However, following exposure to exogenous catecholamine, fluorescent fibers were present in the sweat glands of mature rats and they corresponded in position and density to the plexus localized with acetylcholinesterase staining. Catecholamine uptake was blocked by incubation in the cold and by desmethylimipramine and was not observed in cholinergic parasympathetic fibers in the iris or salivary glands. After intraperitoneal administration of 5-hydroxydopamine and permanganate fixation, all the axons in the sweat glands contained a few SGV. Thus, the developing sweat glands appear to be innervated by noradrenergic axons that lose their stores of endogenous catecholamines but not their capacity for uptake and storage as they elaborte an axonal plexus in the maturing glands. These observations support the hypothesis that cholinergic sympathetic neurons appear to undergo a transition from noradrenergic to cholinergic function during developmentin vivosimilar to that previously described in cell culture.