Breast cancer metastatic potential correlates with a breakdown in homospecific and heterospecific gap junctional intercellular communication.
Breast cancer metastatic potential correlates with a breakdown in homospecific and heterospecific gap junctional intercellular communication.
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发表时间：
2001-03
期刊：
影响因子：
11.2
通讯作者：
M. Saunders;M. Seraj;Zhongyong Li;Zhiyi Zhou;C. Winter;D. Welch;H. Donahue
中科院分区：
文献类型：
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作者：
M. Saunders;M. Seraj;Zhongyong Li;Zhiyi Zhou;C. Winter;D. Welch;H. Donahue
乳腺癌在肿瘤发生和转移阶段朝着越来越恶性的行为发展。在转移阶段的一系列事件中，肿瘤细胞离开乳房中的原发性肿瘤并移动到远处部位，在那里它们建立继发性肿瘤或转移。在这份报告中，我们证明，细胞间的沟通，通过间隙连接恢复在转移性人乳腺癌细胞系MDA-MB-435，当它与乳腺癌转移抑制基因1（BRMS 1）的cDNA转染。此外，间隙连接蛋白（Cxs）的表达谱也发生了变化。具体而言，BRMS 1在MDA-MB-435细胞中的表达增加了Cx43的表达并减少了Cx 32的表达，导致了与正常乳腺组织更相似的间隙连接表型。两者合计，这些结果表明，间隙连接通讯和Cx表达谱可能有助于这些乳腺癌细胞的转移潜力。
Breast cancer progresses toward increasingly malignant behavior in tumorigenic and metastatic stages. In the series of events in the metastatic stage, tumor cells leave the primary tumor in breast and travel to distant sites where they establish secondary tumors, or metastases. In this report, we demonstrate that cell-cell communication via gap junctions is restored in the metastatic human breast carcinoma cell line MDA-MB-435 when it is transfected with breast metastasis suppressor 1 (BRMS1) cDNA. Furthermore, the expression profile of connexins (Cxs), the protein subunits of gap junctions, changes. Specifically, the expression of BRMS1 in MDA-MB-435 cells increases Cx43 expression and reduces Cx32 expression, resulting in a gap junction phenotype more similar to normal breast tissue. Taken together, these results suggest that gap junctional communication and the Cx expression profile may contribute to the metastatic potential of these breast cancer cells.