Changes in intracellular glutathione levels in stimulated and unstimulated lymphocytes in the presence of 2-mercaptoethanol or cysteine.
Changes in intracellular glutathione levels in stimulated and unstimulated lymphocytes in the presence of 2-mercaptoethanol or cysteine.
复制标题
在 2-巯基乙醇或半胱氨酸存在下，刺激和未刺激淋巴细胞的细胞内谷胱甘肽水平发生变化。
作者：
J. Zmuda;B. Friedenson
低分子量。硫醇化合物可以增强各种体外淋巴细胞促有丝分裂反应，而与巯基反应的试剂是有效的细胞毒物。硫醇和硫醇试剂的可能靶点可能包括膜和细胞质蛋白，其中一些可能调节淋巴细胞增殖。作为了解淋巴细胞增殖是如何在硫醇的存在下增强的一步，我们研究了刺激淋巴细胞中蛋白质和非蛋白质巯基的外观。为了帮助澄清巯基的细胞靶点的作用，我们研究了在各种条件下淋巴细胞增殖时蛋白质和非蛋白质巯基出现的时间过程。在适当浓度的巯基2-巯基乙醇（2-ME）的存在下，有丝分裂原伴刀豆球蛋白A（Con A）刺激的淋巴细胞的细胞内谷胱甘肽的水平大幅上升。否则，细胞内谷胱甘肽的水平下降，即使是Con A刺激的细胞，但2-ME的存在部分地防止这种下降。增加培养基中半胱氨酸的量到增强细胞增殖的水平导致与用2-ME获得的效果类似的效果。因此，显然各种硫醇还原剂的一个效果是增强淋巴细胞有丝分裂中谷胱甘肽的产生，并防止静息或增殖淋巴细胞中谷胱甘肽的损失。
Certain low m.w. thiol compounds can enhance various in vitro lymphocyte mitogenic responses, whereas reagents that react with sulfhydryl groups are potent cell poisons. The possible targets for thiols and thiol reagents could include membrane and cytoplasmic proteins, some of which may regulate lymphocyte proliferation. As a step toward understanding how lymphocyte proliferation is enhanced in the presence of thiols, we studied the appearance of both protein and non-protein sulfhydryl groups in stimulated lymphocytes. To help clarify the role of the cellular targets for thiols, we studied the time course of appearance of both protein and non-protein sulfhydryl groups as lymphocytes proliferate under various conditions. In the presence of an appropriate concentration of the thiol 2-mercaptoethanol (2-ME), there is a substantial rise in the level of intracellular glutathione for lymphocytes stimulated with the mitogen concanavalin A (Con A). Otherwise, the level of intracellular glutathione declines, even for Con A-stimulated cells, but the presence of 2-ME partially prevents this decline. Increasing the amount of cysteine in the medium to a level that enhances cell proliferation leads to effects similar to those obtained with 2-ME. Thus, apparently one effect of various thiol reducing agents is to enhance the production of glutathione in lymphocyte mitogenesis and to protect against the loss of glutathione that occurs in resting or proliferating lymphocytes.