Validation of Ultrasonic Harmonic Scalpel for Real-Time Tissue Identification Using Rapid Evaporative Ionization Mass Spectrometry.
Validation of Ultrasonic Harmonic Scalpel for Real-Time Tissue Identification Using Rapid Evaporative Ionization Mass Spectrometry.
复制标题
DOI：
10.1021/acs.analchem.1c00270
复制
发表时间：
2021-04-13
影响因子：
7.4
通讯作者：
Takats Z
中科院分区：
文献类型：
--
作者：
Manoli E;Mason S;Ford L;Adebesin A;Bodai Z;Darzi A;Kinross J;Takats Z
在本研究中，我们将快速蒸发电离质谱（REIMS）与Harmonic超声刀（一种利用超声能量解剖和凝固组织的先进腹腔镜手术器械）相结合。它为外科医生提供了无与伦比的操作能力，并已取代传统的电外科工具，特别是在腹部手术中，但尚未通过REIMS进行验证。通过在负离子和正离子模式下对猪组织进行分析，证明与Harmonic器械配合使用的REIMS平台可产生组织特异性脂质谱。与其他电外科切割方法（如单极电外科和CO2激光）的比较显示，光谱差异取决于所用的能量器械。与单极电外科和CO2激光产生的光谱相比，Harmonic器械在m/z 600-1000的磷脂区域表现出主要光谱差异。在Harmonic REIMS光谱中，观察到甘油二酯和甘油三酯的高强度。相比之下，单极电外科和激光光谱表明甘油磷脂的丰度高。Harmonic超声刀能够区分肝脏、肌肉、结肠和小肠，诊断准确率为100%。Harmonic器械-质谱仪组合的确认将允许平台在各种临床应用中安全可靠地用于实时体内手术组织识别。
In this study, we integrate rapid evaporative ionization mass spectrometry (REIMS) with the Harmonic scalpel, an advanced laparoscopic surgical instrument that utilizes ultrasound energy to dissect and coagulate tissues. It provides unparalleled manipulation capability to surgeons and has superseded traditional electrosurgical tools particularly in abdominal surgery, but is yet to be validated with REIMS. The REIMS platform coupled with the Harmonic device was shown to produce tissue-specific lipid profiles through the analysis of porcine tissues in both negative and positive ionization modes. Comparison with other methods of electrosurgical dissection, such as monopolar electrosurgery and CO2 laser, showed spectral differences in the profile dependent on the energy device used. The Harmonic device demonstrated major spectral differences in the phospholipid region of m/z 600–1000 compared with the monopolar electrosurgical and CO2 laser-generated spectra. Within the Harmonic REIMS spectra, high intensities of diglycerides and triglycerides were observed. In contrast, monopolar electrosurgical and laser spectra demonstrated high abundances of glycerophospholipids. The Harmonic scalpel was able to differentiate between the liver, muscle, colon, and small intestine, demonstrating 100% diagnostic accuracy. The validation of the Harmonic device–mass spectrometry combination will allow the platform to be used safely and robustly for real-time in vivo surgical tissue identification in a variety of clinical applications.
登录
查看更多内容
DOI：
10.1186/s13058-017-0845-2
发表时间：
2017-05-23
期刊：
Breast cancer research : BCR
影响因子：
--
作者：
St John ER;Balog J;McKenzie JS;Rossi M;Covington A;Muirhead L;Bodai Z;Rosini F;Speller AVM;Shousha S;Ramakrishnan R;Darzi A;Takats Z;Leff DR
通讯作者：
Leff DR
影响因子：
8.8
作者：
Phelps DL;Balog J;Gildea LF;Bodai Z;Savage A;El-Bahrawy MA;Speller AV;Rosini F;Kudo H;McKenzie JS;Brown R;Takáts Z;Ghaem-Maghami S
通讯作者：
Ghaem-Maghami S
影响因子：
17.1
作者：
Balog, Julia;Sasi-Szabo, Laszlo;Takats, Zoltan
通讯作者：
Takats, Zoltan
影响因子：
7.4
作者：
Schaefer, Karl-Christian;Balog, Julia;Takats, Zoltan
通讯作者：
Takats, Zoltan
DOI：
10.1007/s00464-016-5121-5
发表时间：
2017-03
期刊：
Surgical endoscopy
影响因子：
--
作者：
Alexander J;Gildea L;Balog J;Speller A;McKenzie J;Muirhead L;Scott A;Kontovounisios C;Rasheed S;Teare J;Hoare J;Veselkov K;Goldin R;Tekkis P;Darzi A;Nicholson J;Kinross J;Takats Z
通讯作者：
Takats Z