Cellulase production on glucose-based media by the UV-irradiated mutants of Trichoderma reesei
Cellulase production on glucose-based media by the UV-irradiated mutants of Trichoderma reesei
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DOI：
10.1007/s00253-010-2683-3
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发表时间：
2010-08-01
影响因子：
5
通讯作者：
Tokuyasu, Ken
中科院分区：
文献类型：
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作者：
Ike, Masakazu;Park, Jeung-yil;Tokuyasu, Ken
从22，791株纤维素酶高产菌株Trichoderma reesei（红褐肉座菌Hypocrea jecorina）ATCC 66589的突变体中，我们选择了两个突变体M2-1和M3-1，它们在含有纤维素和葡萄糖的培养基中产生纤维素酶。突变使突变体产生纤维素酶，这是测量的对硝基苯基β-d-吡喃乳糖苷水解活性，在培养基中与葡萄糖作为唯一的碳源，虽然M2-1表现出不同的敏感性，从M3-1的葡萄糖。当突变株在以纤维素为唯一碳源的培养基上生长8天时，M2-1和M3-1的滤纸降解活性（FPA）分别为257和281 U/g纤维素，是亲本菌株的1.1-1.2倍。M2-1和M3-1在具有葡萄糖和纤维二糖的连续进料混合物的培养基上的纤维素酶生产分别导致214和210 U FPA/克碳源，而亲本菌株实现了较低效率的生产（140 U FPA/克碳源）。突变体纤维素酶生产力的提高使我们能够使用葡萄糖作为碳源，通过质量/数量受控的可溶性碳源和诱导剂补料来有效地现场生产纤维素酶。
From 22,791 mutants of a cellulase hyper-producing strain of Trichoderma reesei (Hypocrea jecorina), ATCC66589, as the parent, we selected two mutants, M2-1 and M3-1, that produce cellulases in media containing both cellulose and glucose. The mutation enabled the mutants to produce cellulases, which were measured as p-nitrophenyl beta-d-lactopyranoside-hydrolyzing activities, in media with glucose as a sole carbon source, although M2-1 exhibited different sensitivities to glucose from M3-1. When the mutants were grown for 8 days on a medium with cellulose as a sole carbon source, the filter-paper-degrading activities (FPAs) per gram of cellulose were 257 and 281 U for M2-1 and M3-1, respectively, values that were 1.1-1.2 times higher than that of the parental strain. Cellulase production by M2-1 and M3-1 on a medium with a continuously fed mixture of glucose and cellobiose resulted in 214 and 210 U of FPA/gram carbon sources, respectively, whereas less efficient production (140 U of FPA/gram carbon source) was achieved by the parental strain. The improved cellulase productivity of the mutants allows us to use glucose as a carbon source for efficient on-site production of cellulases with quality/quantity-controlled feeding of soluble carbon sources and inducers.