Axon regeneration can facilitate or suppress hindlimb function after olfactory ensheathing glia transplantation.
Axon regeneration can facilitate or suppress hindlimb function after olfactory ensheathing glia transplantation.
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DOI：
10.1523/jneurosci.4967-10.2011
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发表时间：
2011-03-16
期刊：
影响因子：
--
通讯作者：
Phelps PE
中科院分区：
文献类型：
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作者：
Takeoka A;Jindrich DL;Muñoz-Quiles C;Zhong H;van den Brand R;Pham DL;Ziegler MD;Ramón-Cueto A;Roy RR;Edgerton VR;Phelps PE
主要基于解剖学证据的报告表明，嗅鞘神经胶质细胞（OEG）移植促进轴突再生在一个完整的脊髓横断在成年大鼠。基于功能，电生理学和解剖学评估，我们发现OEG促进了整个脊髓横断的轴突再生，并且随着时间的推移，这种再生改变了运动反应。在横断后7个月，70%的OEG治疗的大鼠在经颅电刺激后显示后肢肌肉的运动诱发电位。此外，在损伤后8个月进行的完全脊髓再横断表明，这种轴突再生抑制了运动表现，并降低了对机械刺激的过敏性后肢退缩反应。单独OEG移植促进腰骶运动网络的重组，当与长期训练相结合时，增强了一些步进措施。这些新的发现表明，OEG促进成熟轴突的再生和脊髓回路的重组，这两者都有助于感觉运动功能。
Reports based primarily on anatomical evidence suggest that olfactory ensheathing glia (OEG) transplantation promotes axon regeneration across a complete spinal cord transection in adult rats. Based on functional, electrophysiological, and anatomical assessments, we found that OEG promoted axon regeneration across a complete spinal cord transection and that this regeneration altered motor responses over time. At 7 months after transection, 70% of OEG-treated rats showed motor-evoked potentials in hindlimb muscles after transcranial electric stimulation. Furthermore, a complete spinal cord retransection performed 8 months after injury demonstrated that this axon regeneration suppressed locomotor performance and decreased the hypersensitive hindlimb withdrawal response to mechanical stimulation. OEG transplantation alone promoted reorganization of lumbosacral locomotor networks and, when combined with long-term training, enhanced some stepping measures. These novel findings demonstrate that OEG promote regeneration of mature axons across a complete transection and reorganization of spinal circuitry, both of which contribute to sensorimotor function.