The cytological basis of powdery mildew resistance in wild Chinese Vitis species
The cytological basis of powdery mildew resistance in wild Chinese Vitis species
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中国野生葡萄品种白粉病抗性的细胞学基础
DOI：
10.1016/j.plaphy.2019.09.049
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发表时间：
2019-11-01
影响因子：
6.5
通讯作者：
Wen, Ying-Qiang
中科院分区：
文献类型：
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作者：
Hu, Yang;Gao, Yu-Rong;Wen, Ying-Qiang
葡萄白粉病是栽培葡萄的一种重要的经济病害，野生中国葡萄（葡萄属）对葡萄白粉病表现出不同程度的抗性。然而，关于这些野生近缘种的白粉病抗性的细胞学机制的信息很少。在此，我们研究了三种野生中国葡萄材料在感染necator (En) NAFU1后的细胞学反应，并与易感的vinifera cv进行了比较。汤普森无籽葡萄。与“Thompson无核”相比，野生种En NAFU1的菌丝生长和产孢明显受到限制，这似乎与早期细胞壁沉积、吸器被包裹和穿透表皮细胞的超敏反应样细胞死亡有关。此外，在无病原菌条件下，野生葡萄品种的内源游离水杨酸（SA）含量高于汤普森无籽葡萄品种。在En NAFU1定殖过程中，SA结合物在野生葡萄中的积累量高于在Thompson无籽葡萄中的积累量。此外，ennafu1定殖过程中防御相关基因的种特异性表达模式表明，不同野生葡萄品种对白粉病抗性的机制存在差异。这些结果有助于了解野生葡萄品种对白粉病的防御反应机制。
The wild Chinese grapevines (Vitis spp.) show varying levels of resistance to powdery mildew caused by Erysiphe necator that is an economically important disease of cultivated grapevines (Vitis vinifera). However, little information is available regarding the cytological mechanisms of powdery mildew resistance in these wild relatives. Here, we studied the cytological responses of three wild Chinese grapevine accessions after they were infected with E. necator (En) NAFU1 in comparison to the susceptible V. vinifera cv. 'Thompson Seedless' grape. The hyphal growth and sporulation of En NAFU1 were significantly restricted in wild species compared to 'Thompson Seedless', which appears to be associated with early cell wall deposition at the attempt sites, encasement of haustoria, and hypersensitive response-like cell death of penetrated epidermal cells. Moreover, endogenous free salicylic acid (SA) was more abundant in wild Chinese Vitis species than in 'Thompson Seedless' under pathogen-free condition. During En NAFU1 colonization, SA conjugates accumulated higher in wild grapevines than in 'Thompson Seedless'. In addition, the species-specific expression patterns of defense-associated genes during En NAFU1 colonization indicated that mechanisms underlying powdery mildew resistance are divergent among different wild Chinese Vitis species. These results contribute to understanding of mechanisms underlying defense responses of wild Chinese Vitis species against powdery mildew.