Decomposition of Glucose-Sensitive Layer-by-Layer Films Using Hemin, DNA, and Glucose Oxidase
Decomposition of Glucose-Sensitive Layer-by-Layer Films Using Hemin, DNA, and Glucose Oxidase
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DOI：
10.3390/polym12020319
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发表时间：
2019-11
期刊：
影响因子：
5
通讯作者：
Kentaro Yoshida;Yu Kashimura;T. Kamijo;Tetsuya Ono;Takenori Dairaku;Takaya Sato;Yoshitomo Kashiwagi;Katsuhiko Sato
中科院分区：
文献类型：
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作者：
Kentaro Yoshida;Yu Kashimura;T. Kamijo;Tetsuya Ono;Takenori Dairaku;Takaya Sato;Yoshitomo Kashiwagi;Katsuhiko Sato
将氯化高铁血红素修饰的聚乙二亚胺(H-PEI)溶液和含葡萄糖氧化酶(GOx)的DNA溶液逐层沉积，制备了葡萄糖敏感薄膜。利用静电作用制备了H-PEI/DNA+GOx多层膜。(H-PEI/DNA+Gox)5膜被过氧化氢部分分解。LBL膜的分解机制被认为涉及到更多的活性氧物种(ROS)，这些ROS是由氯化高铁血红素和过氧化氢反应形成的，从而导致非特异性DNA切割。此外，LBL膜中的Gox与葡萄糖反应生成过氧化氢。因此，当(H-PEI/DNA+Gox)5薄膜在葡萄糖溶液中浸泡时，可以观察到薄膜的分解。(h-PEI/DNA+Gox)5薄膜在葡萄糖溶液中作用24、48、72和96h后，薄膜的分解率分别增加到9.97%、16.3%、23.1%和30.5%。LBL膜的分解速率随葡萄糖浓度的增加而增加。在接近生理条件的pH和离子强度条件下，可以在1-10 mM的低葡萄糖浓度下缓慢分解LBL膜。
Glucose-sensitive films were prepared through the layer-by-layer (LbL) deposition of hemin-modified poly(ethyleneimine) (H-PEI) solution and DNA solution (containing glucose oxidase (GOx)). H-PEI/DNA + GOx multilayer films were constructed using electrostatic interactions. The (H-PEI/DNA + GOx)5 film was then partially decomposed by hydrogen peroxide (H2O2). The mechanism for the decomposition of the LbL film was considered to involve more reactive oxygen species (ROS) that were formed by the reaction of hemin and H2O2, which then caused nonspecific DNA cleavage. In addition, GOx present in the LbL films reacts with glucose to generate hydrogen peroxide. Therefore, decomposition of the (H-PEI/DNA + GOx)5 film was observed when the thin film was immersed in a glucose solution. (H-PEI/DNA + GOx)5 films exposed to a glucose solution for periods of 24, 48 72, and 96 h indicated that the decomposition of the film increased with the time to 9.97%, 16.3%, 23.1%, and 30.5%, respectively. The rate of LbL film decomposition increased with the glucose concentration. At pH and ionic strengths close to physiological conditions, it was possible to slowly decompose the LbL film at low glucose concentrations of 1–10 mM.