CD9 is a surface marker on mouse and rat male germline stem cells
CD9 is a surface marker on mouse and rat male germline stem cells
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DOI：
10.1095/biolreprod.103.020867
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发表时间：
2004-01-01
影响因子：
3.6
通讯作者：
Shinohara, T
中科院分区：
文献类型：
--
作者：
Kanatsu-Shinohara, M;Toyokuni, S;Shinohara, T
精子发生依赖于少量的干细胞。尽管精原干细胞具有重要的生物学意义，但其稀缺性阻碍了对其的分析。然而，精原干细胞可以通过用抗细胞表面分子的抗体进行选择来富集。在这项研究中，我们寻找新的抗原表达精原干细胞。使用精原细胞移植技术，我们研究了CD9分子的表达，这是通常表达在其他组织的干细胞。小鼠和大鼠睾丸细胞与抗CD9抗体的选择导致精原干细胞从完整的睾丸细胞富集5至7倍，表明CD9通常表达在两个物种的精原干细胞。因此，CD9可能参与许多自我更新组织的干细胞中的共同机制，并且鉴定不同物种的精原干细胞上的共同表面抗原对于开发从其他哺乳动物物种富集干细胞的技术具有重要意义。
Spermatogenesis is dependent on a small population of stem cells. Despite the biological significance of spermatogonial stem cells, their analysis has been hampered by their scarcity. However, spermatogonial stem cells can be enriched by selection with an antibody against cell-surface molecules. In this investigation, we searched for new antigens expressed on spermatogonial stem cells. Using the spermatogonial transplantation technique, we examined expression of the CD9 molecule, which is commonly expressed on stem cells of other tissues. Selection of both mouse and rat testis cells with anti-CD9 antibody resulted in 5- to 7-fold enrichment of spermatogonial stem cells from intact testis cells, indicating that CD9 is commonly expressed on spermatogonial stem cells of both species. Therefore, CD9 may be involved in the common machinery in stem cells of many self-renewing tissues, and the identification of a common surface antigen on spermatogonial stem cells of different species has important implications for the development of a technique to enrich stem cells from other mammalian species.