Two-component sensor required for normal symbiotic colonization of euprymna scolopes by Vibrio fischeri.
Two-component sensor required for normal symbiotic colonization of euprymna scolopes by Vibrio fischeri.
复制标题
费氏弧菌在 Euprymna scolopes 正常共生定植所需的双组件传感器。
DOI：
10.1128/jb.183.3.835-842.2001
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发表时间：
2001
影响因子：
3.2
通讯作者：
Skoufos,LM
中科院分区：
文献类型：
--
作者：
Visick,KL;Skoufos,LM
乌贼的发光器官Euprymna spanopesis特别地被海洋细菌Vibrio fischeri以高密度定殖。到目前为止，只有少数因素有助于这种共生的特异性已被确定。使用遗传筛选缺陷的随机转座子突变体在启动共生协会或殖民轻器官高密度，我们确定了V的突变体。Fischerithat在共生启动中表现出明显的缺陷。该突变体在运动性、发光性或在基本培养基中的生长方面没有缺陷，这表明它缺乏一种必需的、先前未鉴定的共生功能。通过序列分析，我们发现，在这个突变体中失活的基因座编码预测的927个氨基酸的蛋白质具有高度的相似性的传感器组件的混合双组分调控系统。因此，我们指定这个locusrscS，调节器的共生殖民传感器。序列分析显示两个疏水区域，这可能导致参与信号识别的周质环的形成; PhoA融合数据支持这一建议的膜拓扑结构。我们已经通过引物延伸研究了rscS转录的起始位点，并确定了一个假定的启动子区域。我们假设RscS识别与光器官环境相关的信号，并通过刺激控制蛋白质功能或基因表达以协调早期定植事件的假定响应调节剂来响应。对RscS及其同源反应调节因子和信号传导条件的进一步研究将为深入了解V. fischeriandE.我是说...
The light organ of the squidEuprymna scolopesis specifically colonized to a high density by the marine bacteriumVibrio fischeri. To date, only a few factors contributing to the specificity of this symbiosis have been identified. Using a genetic screen for random transposon mutants defective in initiating the symbiotic association or in colonizing the light organ to high density, we identified a mutant ofV. fischerithat exhibited an apparent defect in symbiosis initiation. This mutant was not defective in motility, luminescence, or growth in minimal medium, suggesting that it lacks an essential, previously unidentified symbiotic function. By sequence analysis, we showed that the locus inactivated in this mutant encodes a predicted 927-amino-acid protein with a high degree of similarity to the sensor component of hybrid two-component regulatory systems. We have therefore designated this locusrscS, for regulator of symbiotic colonization—sensor. Sequence analysis revealed two hydrophobic regions which may result in the formation of a periplasmic loop involved in signal recognition; PhoA fusion data supported this proposed membrane topology. We have investigated the start site ofrscStranscription by primer extension and identified a putative promoter region. We hypothesize that RscS recognizes a signal associated with the light organ environment and responds by stimulating a putative response regulator that controls protein function or gene expression to coordinate early colonization events. Further studies on RscS, its cognate response regulator, and the signaling conditions will provide important insight into the interaction betweenV. fischeriandE. scolopes.