Dimethylsulfide is an energy source for the heterotrophic marine bacterium Sagittula stellata
Dimethylsulfide is an energy source for the heterotrophic marine bacterium Sagittula stellata
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DOI：
10.1111/j.1574-6968.2011.02349.x
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发表时间：
2011-09-01
影响因子：
2.1
通讯作者：
Schaefer，Hendrik
中科院分区：
文献类型：
--
作者：
Boden，Rich;Murrell，J. Colin;Schaefer，Hendrik
二甲基硫化物(DMS)是一种挥发性有机硫化合物，广泛存在于海洋中，被认为在生物地球化学循环和气候控制中发挥着各种作用。DMS的海洋下沉是已知的--既有化学的，也有生物的--尽管人们对它们知之甚少。除了利用DMS作为碳源或硫源外，一些细菌还能将其氧化成二甲基亚砜(DMSO)。星形箭虫是海洋环境中发现的甲型保护性细菌的异养成员。在糖的异养生长过程中，它被证明氧化DMS，但这种氧化的原因和机理还没有被研究。在这里，我们证明了DMS的氧化为DMSO是与ATP合成INS偶联的。DMS在有机体以果糖和琥珀酸为底物的化学有机异养生长过程中起到能量来源的作用。在DMS存在下生长的细胞中不存在DMS脱氢酶(在其他海洋细菌中负责将DMS氧化为DMSO)和DMSO还原酶活性，这表明该生物体中DMS氧化的另一种途径。
Dimethylsulfide (DMS) is a volatile organosulfur compound, ubiquitous in the oceans, that has been credited with various roles in biogeochemical cycling and in climate control. Various oceanic sinks of DMS are known – both chemical and biological – although they are poorly understood. In addition to the utilization of DMS as a carbon or a sulfur source, someBacteriaare known to oxidize it to dimethylsulfoxide (DMSO).Sagittula stellatais a heterotrophic member of theAlphaproteobacteriafound in marine environments. It has been shown to oxidize DMS during heterotrophic growth on sugars, but the reasons for and the mechanisms of this oxidation have not been investigated. Here, we show that the oxidation of DMS to DMSO is coupled to ATP synthesis inS. stellataand that DMS acts as an energy source during chemoorganoheterotrophic growth of the organism on fructose and on succinate. DMS dehydrogenase (which is responsible for the oxidation of DMS to DMSO in other marineBacteria) and DMSO reductase activities were absent from cells grown in the presence of DMS, indicating an alternative route of DMS oxidation in this organism.