Determination of polyphenolic compounds in commercial herbal drugs and spices from Lamiaceae: thyme, wild thyme and sweet marjoram by chromatographic techniques
Determination of polyphenolic compounds in commercial herbal drugs and spices from Lamiaceae: thyme, wild thyme and sweet marjoram by chromatographic techniques
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DOI：
10.1016/j.foodchem.2007.11.035
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发表时间：
2008-06-01
期刊：
影响因子：
8.8
通讯作者：
Turek, Sebastian
中科院分区：
文献类型：
--
作者：
Fecka, Izabela;Turek, Sebastian
采用高效液相色谱(HPTLC)和高效液相色谱(HPTLC)法，定性和定量测定了百里香、蛇床子草和百合香料中木犀草素-7-O-β-葡萄糖醛酸脂、石香酸、迷迭香酸和迷迭香酸甲酯等已知化合物的含量。采用C18色谱柱，以乙腈-水-甲酸为流动相，测定了所研究的中草药来源的亲水性提取物中被分析化合物的含量。对所建立的方法进行了线性、精密度和准确度验证。其中木犀草素-7-O-β-葡萄糖醛酸苷和石香酸为新的野生百里香成分，木犀草素-7-O-β-葡萄糖醛酸脂和迷迭香酸甲酯为新化合物。迷迭香酸甲酯为首次从百里香中分离得到。所研究的草药和香料提供了大量的多酚，甚至高达每1克干燥草本植物84.3毫克。(C)2007爱思唯尔有限公司。保留所有权利。
HPLC and HPTLC methods were used for a qualitative and quantitative determination of luteolin-7-O-beta-glucuronide, lithospermic acid, rosmarinic acid and mthyl rosmarinate, together with other known compounds, in commercial herbal drugs and spices from lamiaceous species: Thymi herba, Serpylli herba and Majoranae herba. The contents of analyzed compounds in the studied hydrophilic extracts, prepared form herbal sources, were established using a C18 column with acetonitrile-water-formic acid as a mobile phase. The HPLC method was validated for linearity, precision and accuracy. Luteolin-7-O-beta-glucuronide and lithospermic acid were identified as new wild thyme constituents, luteolin-7-O-beta-glueuronide and methyl rosmarinate as new compounds in sweet marjoram. Methyl rosmarinate was isolated for the first time from thyme. The investigated herbal drugs and spices provide polyphenols in high amounts, even up to 84.3 mg per 1 g of a dried herb. (C) 2007 Elsevier Ltd. All rights reserved.