INDEPENDENT REGULATION OF CALCIUM REVEALED BY IMAGING DENDRITIC SPINES
INDEPENDENT REGULATION OF CALCIUM REVEALED BY IMAGING DENDRITIC SPINES
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DOI：
10.1038/354076a0
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发表时间：
1991-11-07
期刊：
影响因子：
64.8
通讯作者：
KATER, SB
中科院分区：
文献类型：
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作者：
GUTHRIE, PB;SEGAL, M;KATER, SB
树突棘是神经元组织的基本结构单位。它被认为是突触可塑性的主要位点，并经历长期的形态和功能变化1 - 6，其中至少有一些是由细胞内钙浓度调节7 - 11。已知生理刺激可引起海马树突轴中细胞内钙水平的显著增加12、13，但完全不知道树突中的这种变化在多大程度上也会被棘内的钙敏感结构看到。所有棘中的钙水平是否与树突平行变化，或者是否存在异质反应？这项研究，通过直接可视化和测量的细胞内钙浓度在个人生活的脊椎，表明实验诱发的钙浓度的变化在树突状干（[Ca2 +] d）往往不是平行的脊椎（[Ca2 +] s）。这种隔离不是由物理扩散势垒引起的。据我们所知，这份报告提供了自主脊柱功能的第一个直接证明。
THE dendritic spine is a basic structural unit of neuronal organization. It is assumed to be a primary locus of synaptic plasticity, and to undergo long-term morphological and functional changes 1-6, at least some of which are regulated by intracellular calcium concentrations 7-11. It is known that physiological stimuli can cause marked increases in intracellular calcium levels in hippocampal dendritic shafts 12,13, but it is completely unknown to what extent such changes in the dendrites would also be seen by calcium-sensing structures within spines. Will calcium levels in all spines change in parallel with the dendrite or will there be a heterogeneous response? This study, through direct visualization and measurement of intracellular calcium concentrations in individual living spines, demonstrates that experimentally evoked changes in calcium concentrations in the dendritic shaft ([Ca2+]d) are frequently not parallelled in the spine ([Ca2+]s). This isolation is not caused by a physical diffusion barrier. This report provides, to our knowledge, the first direct demonstration of autonomous spine function.