Effect of tooth loss on spatial memory and trkB‐mRNA levels in rats
Effect of tooth loss on spatial memory and trkB‐mRNA levels in rats
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DOI：
10.1002/hipo.20440
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发表时间：
2008-06
期刊：
影响因子：
3.5
通讯作者：
K. Yamazaki;N. Wakabayashi;Takuya Kobayashi;Tetsuya Suzuki
中科院分区：
文献类型：
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作者：
K. Yamazaki;N. Wakabayashi;Takuya Kobayashi;Tetsuya Suzuki
牙齿脱落加速空间记忆障碍的机制尚不清楚。本研究的目的是验证牙齿脱落影响trkB‐mRNA水平并导致大鼠海马细胞密度加速下降的假设。采用径向迷宫法评价雄性Wistar大鼠的空间记忆能力。利用分子生物学技术评估海马锥体细胞的数量和杏仁核、周围皮层、丘脑和海马CA1、CA3和CA4区trkB‐mRNA的表达。拔牙7周后，各缺牙组的迷宫表现明显低于对照组，拔牙数量与trkB‐mRNA诱导量和海马细胞密度成反比。在整个实验过程中，通过控制喂养，大鼠的平均体重都有所增加，但对照组和实验组之间没有明显差异。结果表明，在大鼠中，空间记忆相关的trkB - mRNA与牙齿脱落相关；这支持了这一假设，并表明牙齿在预防空间记忆障碍方面发挥了作用。©2008 Wiley‐Liss, Inc。
The mechanism by which tooth loss accelerates spatial memory impairment is unknown. The purpose of this study was to test the hypothesis that tooth loss affects trkB‐mRNA levels and leads to an accelerated decrease in the hippocampal cell density in rats. A radial maze was used to evaluate the spatial memory of male Wistar rats that were categorized based on the number of extracted molar teeth. Number of hippocampal pyramidal cells and the trkB‐mRNA expressions in the amygdala, perirhinal cortex, thalamus, and the hippocampal CA1, CA3, and CA4 areas, were evaluated using molecular biological techniques. Seven weeks after tooth extraction, maze performance was significantly lower in each tooth loss group than in the control group, and the number of extracted teeth was inversely proportional to the induction of the trkB‐mRNA and the hippocampal cell density. The average weight of rats increased by controlled feeding throughout the experiment without showing a significant difference between the control and experimental groups. The results indicated that, in rats, the spatial memory‐linked trkB‐mRNA was reduced in association with the tooth loss; this supports the hypothesis and suggests that teeth have a role in the prevention of spatial memory impairment. © 2008 Wiley‐Liss, Inc.