Streptomyces genes involved in aerial mycelium formation

Streptomyces genes involved in aerial mycelium formation
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链霉菌基因参与气生菌丝体形成

DOI:
10.1111/j.1574-6968.1996.tb08355.x
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发表时间:
1996
影响因子:
2.1
通讯作者:
S. Horinouchi
S. Horinouchi
中科院分区:
生物学4区
文献类型:
--
作者:
S. Horinouchi

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作为抑制剂的气生菌丝体缺陷型的灰色链霉菌HH 1从A-因子缺陷的基因的克隆导致了几个基因的鉴定,包括amfR,amfAlamfB,amfC,和orf 1590。这些基因参与气生菌丝体的形成,而不依赖于次生代谢功能。其中,AmfR属于双组分调节系统的反应调节子家族,AmfA/AmfB类似于ATP依赖性膜转位子,分别类似于多组分磷酸化中继和Spo 0 K系统,这两者都是枯草芽孢杆菌孢子形成起始所需的。还表明蛋白质丝氨酸/苏氨酸激酶参与气生菌丝体形成,因为天蓝色链霉菌A3(2)afsK基因编码“真核”型蛋白激酶,逆转菌株HH 1的气生菌丝体缺陷表型,不依赖于次级代谢功能。
Cloning of genes as suppressors of the aerial mycelium-defective phenotype of Streptomyces griseus HH1 resulting from A-factor-deficiency has led to the identification of several genes, including amfR, amfAlamfB, amfC, and orf1590. These genes are involved in aerial mycelium formation independent of secondary metabolic function. Among these, AmfR which belongs to the family of response regulators of two-component regulatory systems and AmfA/AmfB similar to ATP-dependent membrane translocators are analogous to the multicomponent phosphorelay and the Spo0K system, respectively, both of which are required for the initiation of sporulation in Bacillus subtilis. Involvement of a protein serine/threonine kinase in aerial mycelium formation is also suggested, because the Streptomyces coelicolor A3(2) afsK gene encoding a ‘eukaryotic’-type protein kinase reverses the aerial mycelium-defective phenotype of strain HH1, independent of secondary metabolic function.
S.Horinouchi:“次生代谢和细胞分化的调节是链霉菌:A 因子作为微生物激素和 AfsR 蛋白作为双组分调节系统的组成部分”基因。
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DOI: 10.1128/jb.175.9.2652-2661.1993
发表时间: 1993-05
影响因子: 3.2
作者:
D. Vujaklija;S. Horinouchi;T. Beppu
通讯作者: D. Vujaklija;S. Horinouchi;T. Beppu
K.Ueda:“灰色链霉菌中参与气生菌丝体形成的基因簇编码与双组分调节系统和膜易位子的反应调节器相似的蛋白质”J.Bacteriol.2006-2016 (1993)
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