Selection and Evaluation of Indexes Commonly Used to Determine Contamination with T-2 Toxin in Pacific White Shrimp Litopenaeus vannamei by the Grey Relational Method
Selection and Evaluation of Indexes Commonly Used to Determine Contamination with T-2 Toxin in Pacific White Shrimp Litopenaeus vannamei by the Grey Relational Method
复制标题
灰色关联法筛选和评价凡纳滨对虾T-2毒素污染常用指标
DOI：
10.1080/08997659.2017.1320318
复制
发表时间：
2017
影响因子：
1.2
通讯作者：
Bi Siyuan
中科院分区：
文献类型：
--
作者：
Lu Pengli;Wang Yaling;Dai Zhe;Sun Lijun;Xu Defeng;Liu Ying;Ye Riying;Gooneratne Ravi;Bi Siyuan
研究饲料中添加不同浓度的真菌毒素T-2毒素对凡纳滨对虾肌肉生产性能的影响，并采用灰色关联分析法评价T-2毒素对凡纳滨对虾肌肉生产性能的影响，确定T-2毒素与凡纳滨对虾肌肉生产性能的剂量-反应关系。在所检测的6项生理指标、7项生化指标和17项营养指标中，肝胰脏的体重比、丙氨酸氨基转移酶（ALT）活性和丝氨酸（SER）含量的灰色关联系数最高，分别为0.83、0.68和0.82。因此，选择HBR、ALT活性和SER含量作为T-2毒素污染太平洋白色对虾肌肉的适宜指标。根据其剂量-反应关系曲线，获得的HBR、ALT活性和SER含量的平均有效剂量分别为1.45、1.69和1.33 mg T-2毒素/kg饲料。这些结果为虾饲料中T‐2毒素的评价和控制提供了技术参考点。接收时间：2016年4月28日接受日期：2017年4月9日在线发布日期：2017年7月26日
The objectives of the present study were to evaluate the effects of different concentrations of the mycotoxin T‐2 toxin in feed on muscle performance in the Pacific white shrimpLitopenaeus vannamei, evaluate indexes of physiological variables that indicate T‐2 toxin contamination in the shrimp using the grey relational method, and determine the dose–response relationships between T‐2 toxin and the indexes. Of the 6 physical, 7 biochemical, and 17 nutritional indexes examined, the values of the grey relational coefficients were highest for the hepatopancreas: body weight ratio (HBR), alanine aminotransferase (ALT) activity, and serine (SER) content (0.83, 0.68, and 0.82, respectively). Therefore, the HBR, ALT activity, and SER content were selected as appropriate indexes for contamination of Pacific white shrimp muscle with T‐2 toxin. Based on their dose–response relationship curves, mean effective doses of 1.45, 1.69, and 1.33 mg of T‐2 toxin/kg of feed were obtained for the HBR, ALT activity, and SER content, respectively. These results offer technical reference points for the evaluation and control of T‐2 toxin in shrimp feed.Received April 28, 2016 accepted April 9, 2017 Published online July 26, 2017