Molecular cloning of cDNA for cholesterol 7 alpha-hydroxylase from rat liver microsomes. Nucleotide sequence and expression.
Molecular cloning of cDNA for cholesterol 7 alpha-hydroxylase from rat liver microsomes. Nucleotide sequence and expression.
复制标题
大鼠肝微粒体胆固醇 7 α-羟化酶 cDNA 的分子克隆。
DOI：
10.1016/0014-5793(89)81795-8
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发表时间：
1989
期刊：
影响因子：
3.5
通讯作者：
K. Okuda
中科院分区：
文献类型：
--
作者：
M. Noshiro;M. Nishimoto;K. Morohashi;K. Okuda
利用胆固醇7α-羟化酶的特异性抗体，从大鼠肝cDNA文库中克隆了一个编码胆固醇7α-羟化酶的完整cDNA克隆。该cDNA克隆全长3.6 kbp，含有一个1509 bp的开放阅读框，编码503个氨基酸残基（Mr= 56880）。用pSVL表达载体转染COS细胞，经胆固醇7α-羟化酶活性和免疫反应性蛋白表达证实了cDNA的同一性。胆固醇7 α-羟化酶;细胞色素P-450; cDNA克隆;（COS细胞）
A complete cDNA clone encoding cholesterol 7α-hydroxylase was isolated from a rat liver cDNA library by the use of specific antibodies to the enzyme. The isolated cDNA clone was 3.6 kbp long and contained a 1509-bp open reading frame encoding 503 amino acid residues (Mr= 56 880). The identity of the cDNA was confirmed by expression of cholesterol 7α-hydroxylase activity and the immunoreactive protein in COS cells transfected with pSVL expression vector carrying the cDNA insert. The primary structure of cholesterol 7α-hydroxylase deduced from the nucleotide sequence of the cDNA indicated that the enzyme constitutes a novel P-450 family.Cholesterol 7α-hydroxylase; Cytochrome P-450; cDNA cloning; (COS cell)
DOI：
10.1073/pnas.84.14.4767
发表时间：
1987-07-01
影响因子：
11.1
作者：
TABOR, S;RICHARDSON, CC
通讯作者：
RICHARDSON, CC
影响因子：
56.9
作者：
M. X. Zuber;E. Simpson;M. Waterman
通讯作者：
M. X. Zuber;E. Simpson;M. Waterman
DOI：
10.1073/pnas.80.5.1194
发表时间：
1983-01-01
期刊：
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES
影响因子：
--
作者：
YOUNG, RA;DAVIS, RW
通讯作者：
DAVIS, RW