Improvement of pharmacokinetic and antitumor activity of layered double hydroxide nanoparticles by coating with PEGylated phospholipid membrane.
Improvement of pharmacokinetic and antitumor activity of layered double hydroxide nanoparticles by coating with PEGylated phospholipid membrane.
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聚乙二醇化磷脂膜包覆层状双氢氧化物纳米粒子的药代动力学和抗肿瘤活性的改善
DOI：
10.2147/ijn.s69729
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发表时间：
2014
影响因子：
8
通讯作者：
Zhao C
中科院分区：
文献类型：
--
作者：
Yan M;Zhang Z;Cui S;Lei M;Zeng K;Liao Y;Chu W;Deng Y;Zhao C
层状双氢氧化物（LDH）作为药物载体受到了广泛关注。然而，由于其较差的体内行为，聚乙二醇化（PEG化）磷脂必须用作共形成剂以产生自组装核-壳纳米颗粒。在本研究中，我们制备了聚乙二醇化磷脂包被的LDH（PLDH）（PEG-PLDH）传递系统。PEG-PLDH纳米颗粒具有133.2nm的平均尺寸。通过透射电子显微镜和X射线光电子能谱证实了它们的核壳结构。体外脂质体-细胞缔合和细胞毒性实验证明了其被细胞内化的能力。体内研究表明，聚乙二醇化磷脂膜大大降低了LDH纳米粒的血液清除率。PEG-PLDH纳米颗粒表现出对肿瘤生长的良好控制，并提高了小鼠的存活率。这些结果表明，PEG-PLDH纳米粒子可以是一个有用的药物输送系统的癌症治疗。
Layered double hydroxide (LDH) has attracted considerable attention as a drug carrier. However, because of its poor in vivo behavior, polyethylene glycolylated (PEGylated) phospholipid must be used as a coformer to produce self-assembled core–shell nanoparticles. In the present study, we prepared a PEGylated phospholipid-coated LDH (PLDH) (PEG-PLDH) delivery system. The PEG-PLDH nanoparticles had an average size of 133.2 nm. Their core–shell structure was confirmed by transmission electron microscopy and X-ray photoelectron spectroscopy. In vitro liposome-cell-association and cytotoxicity experiments demonstrated its ability to be internalized by cells. In vivo studies showed that PEGylated phospholipid membranes greatly reduced the blood clearance rate of LDH nanoparticles. PEG-PLDH nanoparticles demonstrated a good control of tumor growth and increased the survival rate of mice. These results suggest that PEG-PLDH nanoparticles can be a useful drug delivery system for cancer therapy.