Stiffness measurement of organoids using a wide-range force sensor probe fabricated using a quartz crystal resonator
Stiffness measurement of organoids using a wide-range force sensor probe fabricated using a quartz crystal resonator
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使用由石英晶体谐振器制造的宽范围力传感器探头测量类器官的刚度
DOI：
10.1109/lra.2022.3144766
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发表时间：
2022
期刊：
影响因子：
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通讯作者：
Hirotaka Sugiura and Fumihito Arai
中科院分区：
文献类型：
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作者：
Shiro Watanabe;Hirotaka Sugiura and Fumihito Arai
为了筛选生物和化学领域的化学物质，需要实现实验过程和评价方法的自动化。在生物评价的情况下，考虑了机械探测的自动化，但由于生物样品的力学性质分布广泛，测量方法应具有通用性。我们提出了一种使用石英晶体谐振器(QCR)制作力传感器探头来测量刚度的方法。由于存在低频噪声，QCR力传感器具有较宽的测力范围。我们测量了具有不同杨氏模数的两个样品，有机化合物和聚二甲基硅氧烷(PDMS)微珠的硬度。所制作的QCR传感器探头的测量范围超过5位数，力分辨率为272nN，最大载荷大于30mN。利用传感器探头成功地测量了有机微珠和PDMS微珠的硬度，分别为2.51 kPa和1.19 Mpa。结果，我们成功地测量了杨氏模数分布在三位数以上的各种试件的刚度。
The automation of the experimental process and evaluation method is required to screen chemical substances in the fields of biology and chemistry. In the case with biological evaluations, the automation of the mechanical probing has been considered, but the measurement method should possess versatility as the mechanical properties of biological samples widely distribute. We present a stiffness measurement using a quartz crystal resonator (QCR) fabricated force sensor probe. Owing to the low frequency noise, the QCR force sensor exhibits a wide force measurement range. We measured the stiffness of the two samples, organoids and polydimethylsiloxane (PDMS) beads, that have different Young’s modulus. The fabricated QCR sensor probe exhibited the measurement range over five digits with the force resolution of 272 nN and the maximum load of over 30 mN. Using the sensor probe, the stiffness of organoids and PDMS beads was successfully evaluated; 2.51 kPa and 1.19 MPa, respectively. As a result, we succeeded in measuring the stiffness of various samples whose Young’s modulus distributed over three digits.