IDENTIFICATION OF 2 DISTINCT SYNUCLEINS FROM HUMAN BRAIN
IDENTIFICATION OF 2 DISTINCT SYNUCLEINS FROM HUMAN BRAIN
复制标题
DOI：
10.1016/0014-5793(94)00395-5
复制
发表时间：
1994-05-23
期刊：
影响因子：
3.5
通讯作者：
GOEDERT, M
中科院分区：
文献类型：
--
作者：
JAKES, R;SPILLANTINI, MG;GOEDERT, M
从人脑中分离纯化了两种富含140和134个氨基酸的蛋白质，并对其进行了序列测定。通过它们在免疫印迹上与部分特征的单抗的反应来鉴定它们，该单抗以磷酸化依赖的方式识别tau蛋白。140个氨基酸的蛋白质与阿尔茨海默病淀粉样蛋白的非AB成分的前体相同，而后者又与来自鱼雷电斑和大鼠大脑的突触核蛋白高度同源。134个氨基酸的蛋白质是人的牛磷酸神经蛋白14的同源物；它的序列与140个氨基酸的蛋白质有61%的同源性。这两个蛋白质之间以前未被认识到的同源性定义了一个人类大脑突触核蛋白家族。我们将140和134个氨基酸的蛋白质分别称为α-突触核蛋白和β-突触核蛋白。这两种突触核蛋白主要在大脑中表达，它们集中在突触前神经末梢。
Two abundant proteins of 140 and 134 amino acids were purified and sequenced from human brain. They were identified through their reactivity on immunoblots with a partially characterised monoclonal antibody that recognises tau protein in a phosphorylation-dependent manner. The 140 amino acid protein is identical with the precursor of the non-AB component of Alzheimer's disease amyloid which in turn is highly homologous to synuclein from Torpedo electroplaques and rat brain. The 134 amino acid protein is the human homologue of bovine phosphoneuroprotein 14; it is 61% identical in sequence to the 140 amino acid protein. The previously unrecognised homology between these two proteins defines a family of human brain synucleins. We refer to the 140 and 134 amino acid proteins as alpha-synuclein and beta-synuclein, respectively. Both synucleins are expressed predominantly in brain, where they are concentrated in presynaptic nerve terminals.