Marginal periodontium as a potential reservoir of human papillomavirus in oral mucosa
Marginal periodontium as a potential reservoir of human papillomavirus in oral mucosa
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DOI：
10.1902/jop.2005.76.3.358
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发表时间：
2005-03-01
影响因子：
4.3
通讯作者：
Syrjänen, S
中科院分区：
文献类型：
--
作者：
Hormia, M;Willberg, J;Syrjänen, S
背景：虽然人类乳头瘤病毒(HPV)与包括鳞状细胞恶性肿瘤在内的许多增生性上皮病变有关，但在10%至20%的成年人正常口腔粘膜中也可检测到HPVs。然而，HPV在口腔中的入口点和复制位置尚不清楚。由于在口腔粘膜中，基底层细胞通常是HPV在其他粘膜部位的靶细胞，因此我们推测这可能是潜伏HPV的部位。方法：对38例临床诊断为牙周病的患者进行了牙龈活检。应用巢式聚合酶链式反应(针对HPV L1区的MY09/MY11和GP05+/GP06+引物)检测HPVDNA的存在，然后用12个高危型HPV寡核苷酸与筛查HPV和HPV16亚型的探针进行原位杂交。结果：在本研究中，26%(8/31)的牙龈活检组织中检测到高危型HPV。结论：牙周袋可能是口腔粘膜中HPV的储存区。虽然这对理解HPV的传播有重要的意义，但这一观察不排除HPV可能参与牙周病的发生。
Background: Although human papillomaviruses (HPVs) are associated with a number of proliferative epithelial lesions including squamous cell malignancies, they can also be detected in the normal oral mucosa in 10% to 20% of the adult population. However, the point of entry and the site of replication of HPV in the oral cavity are not known. Since the gingival pocket is the only site in the oral mucosa where basal cells, known to be targets of HPV at other mucosal sites, are normally exposed to the environment, we hypothesized that this could be the site of latent HPV.Methods: Gingival biopsies taken from 38 individuals with clinically diagnosed periodontal disease were examined. The presence of HPV DNA was studied by using nested PCR (polymerase chain reaction with MY09/MY11 and GP05+/GP06+ primers targeting the L1 region of HPV), followed by subsequent hybridization with a cocktail of 12 high-risk HPV oligoprobes and in situ hybridization (ISH) with probes for HPV screening and the HPV subtype 16.Results: In the present study, high-risk HPV types were detected in 26% (8/31) of the gingival biopsies with PCR. By using in situ hybridization, the viral DNA was localized to the coronal part of the junctional epithelium in the gingival pocket.Conclusions: The results suggest that the periodontal pocket might serve as a reservoir of HPVs in oral mucosa. While having important implications in understanding the HPV transmission, this observation does not rule out the possibility that HPV may be involved in the initiation of periodontal disease.