The mutated SI-haplotype in sour cherry has an altered S-haplotype-specific F-box protein gene
The mutated SI-haplotype in sour cherry has an altered S-haplotype-specific F-box protein gene
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DOI：
10.1093/jhered/esl029
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发表时间：
2006-09-01
影响因子：
3.1
通讯作者：
Iezzoni, Amy F.
中科院分区：
文献类型：
--
作者：
Hauck, Nathanael R.;Ikeda, Kazuo;Iezzoni, Amy F.
配子体自交不亲和性（GSI）是显花植物的一种异交机制，它由位于高度多态性S位点的两个独立基因控制，称为S单倍型。本研究描述了酸樱桃（Rosaceae，Prunus cerevisiaL.）导致了GSI的消失以S-1突变单倍型（S-1'S4 SdSnull）的酸樱桃自交亲和品种Ujfehertoi Furtos和甜樱桃自交不亲和品种Prunus avium L.携带野生型S-1单倍型的栽培品种揭示突变的S-1单倍型赋予功能雌蕊组分和非功能花粉组分的单侧不亲和性。突变的酸樱桃S-1单倍型花粉部分突变体（S-1 '）在S-1单倍型特异的F-box蛋白基因（SFB 1'）中含有一个615 bp的Ds-like元件。这种插入产生了一个过早的框内终止密码子，这将导致一个推定的截短的SFB 1只含有75的375个氨基酸存在于野生型SFB 1。S-1'沿着另外两个先前鉴定的李属S-单倍型突变体S-f和S-6 m，说明移动的元件插入是GSI分解的进化动力。
Gametophytic self-incompatibility (GSI) is an outcrossing mechanism in flowering plants that is genetically controlled by 2 separate genes located at the highly polymorphic S-locus, termed S-haplotype. This study characterizes a pollen part mutant of the S-1-haplotype present in sour cherry (Rosaceae, Prunus cerasus L.) that contributes to the loss of GSI. Inheritance of S-haplotypes from reciprocal interspecific crosses between the self-compatible sour cherry cultivar Ujfehertoi Furtos carrying the mutated S-1-haplotype (S-1' S4SdSnull) and the self-incompatible sweet cherry (Prunus avium L.) cultivars carrying the wild-type S-1-haplotype revealed that the mutated S-1-haplotype confers unilateral incompatibility with a functional pistil component and a nonfunctional pollen component. The altered sour cherry S-1-haplotype pollen part mutant, termed S-1', contains a 615-bp Ds-like element within the S-1-haplotype-specific F-box protein gene (SFB1'). This insertion generates a premature in-frame stop codon that would result in a putative truncated SFB1 containing only 75 of the 375 amino acids present in the wild-type SFB1. S-1' along with 2 other previously characterized Prunus S-haplotype mutants, S-f and S-6m, illustrate that mobile element insertion is an evolutionary force contributing to the breakdown of GSI.