Effects of Shenlian Extracts (sic) on Atherosclerosis by Inhibition of the Inflammatory Response
Effects of Shenlian Extracts (sic) on Atherosclerosis by Inhibition of the Inflammatory Response
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参连提取物（原文如此）通过抑制炎症反应对动脉粥样硬化的影响
DOI：
10.1016/s0254-6272(12)60016-8
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发表时间：
2011-12-01
影响因子：
2.6
通讯作者：
Zhu Xiao-xin
中科院分区：
文献类型：
--
作者：
Li Yu-jie;Chen Ying;Zhu Xiao-xin
目的：炎症在动脉粥样硬化中起着至关重要的作用，这种炎症反应正在得到深入研究。参连提取物（原文如此）是一种中药活性成分，被认为对包括动脉粥样硬化在内的人类血管疾病具有多种治疗和预防作用。我们的工作研究了参连提取物是否在动脉粥样硬化形成过程中发挥抗炎作用。方法：我们通过球囊血管成形术和高胆固醇饮食建立了兔子动脉粥样硬化模型。通过股动脉苏木精-伊红染色、血清或血管组织中炎症相关因子的测定以及放射免疫分析来评估参连提取物对血管结构和炎症的影响。还进行了酶联免疫吸附测定（ELISA）、流式细胞术和蛋白质印迹。结果：我们表明，口服参连提取物预处理可抑制与动脉粥样硬化相关的病理变化，并且分级剂量的参连提取物可降低血清总胆固醇（90、180和360毫克/千克）、甘油三酯（180和360毫克/千克）和低密度脂蛋白胆固醇（90、180和360毫克/千克）的总血清水平。 180毫克/公斤）。不同剂量的参连提取物降低血清中TNF-α（180和360 mg/kg）、CRP（90、180和360 mg/kg）和IL-8（360 mg/kg）的含量（P
Objective: Inflammation plays a critical role in atherosclerosis, and this inflammatory reaction is being intensively studied. Shenlian Extracts (sic), an active ingredient of Chinese medicinal herbs, is believed to have multiple therapeutic and preventive effects against human vascular diseases, including atherosclerosis. Our work investigated whether Shenlian Extracts serves as an anti-inflammatory agent during atherogenesis.Methods: We established a model of atherosclerosis in rabbits using balloon angioplasty and a high cholesterol diet. The effects of Shenlian Extracts on vessel structure and inflammation were assessed by hematoxylin-eosin staining of the femoral artery, measurement of inflammation-related factors in serum or vascular tissue, and radioimmunoassay. Enzyme linked immunosorbent assays (ELISA), flow cytometry and western blots were also performed.Results: We show that oral pre-treatment with Shenlian Extracts suppressed the pathological changes associated with atherosclerosis and that graded doses of Shenlian Extracts reduced total serum levels of cholesterol (90, 180 and 360 mg/kg), triglyceride (180 and 360 mg/kg), and LDL-c (90, 180 mg/kg). Various doses of Shenlian Extracts reduced serum content of TNF-alpha (180 and 360 mg/kg), CRP (90, 180 and 360 mg/kg) and IL-8 (360 mg/kg) (P